Strain Data Sheet
RBRC01105
Strain Information | |
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| Image | |
| BRC No. | RBRC01105 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.Cg-Sema3a<tm1.3Tyag>/TyagRbrc |
| Former Common name | semaphorin III/D (lacZ) Targeting mice |
| H-2 Haplotype | |
| ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
| Background strain | C57BL/6NCrlCrlj |
| Appearance | black [a/a B/B C/C] |
| Strain development | Developed by Masahiko Taniguchi and Takeshi Yagi, National Institute for Physiological Sciences in 1997. TT2 ES cells were used. The mutant mice were backcrossed to C57BL/6 over 5 times. |
| Strain description | Semaphorin III/D (Sema3a) knockout mice (lacZ reporter). Most of homozygous mice in C57BL/6 genetic background are lethal. About one-tenth of the homozygous mutant mice of ICR background survived (Depositor, personal communication). |
| Colony maintenance | Heterozygote x Wild-type [or Crossing to C57BL/6NCrlCrlj] |
| References | Disruption of semaphorin III/D gene causes severe abnormality in peripheral nerve projection. Taniguchi M, Yuasa S, Fujisawa H, Naruse I, Saga S, Mishina M, Yagi T Neuron, 19, 519-530 (1997). 9331345 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Sema3a | sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A | 5 | Sema3a | targeted mutation 1.3, Takeshi Yagi | |||
| lacZ | beta-galactosidase (E. coli) | 5 | |||||
| loxP | phage P1 loxP | 5 | loxP | ||||
| loxP | phage P1 loxP | 5 | loxP | ||||
| neo | neomycin resistance gene (E. coli) | 5 | mouse phosphoglycerate kinase promoter (PGK promoter) | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | phage P1 loxP sites, E. coli lacZ, SV40 early pA signal, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, poly A, mouse semaphorin 3A genomic DNA |
| Research application | Cre/loxP system Fluorescent Proteins/lacZ System Neurobiology Research |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuron, 19, 519-530 (1997).Any patent rights and other intellectual properties are retained by the depositor. Users have to contact the depositor in the case of application for any patents with the result from these mice. When users releases research results that obtained in two years after deposition, users should contact a bailor about collaboration. |
| Depositor | Takeshi Yagi (National Institute for Physiological Sciences) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Aug 2006 |
BRC mice in Publications |
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Homman-Ludiye J, Bourne JA. The guidance molecule Semaphorin3A is differentially involved in the arealization of the mouse and primate neocortex. Cereb Cortex 24(11) 2884-98(2014) 23709645 |
Cioni JM, Telley L, Saywell V, Cadilhac C, Jourdan C, Huber AB, Huang JZ, Jahannault-Talignani C, Ango F. SEMA3A signaling controls layer-specific interneuron branching in the cerebellum. Curr Biol 23(10) 850-61(2013) 23602477 |
Zhou J, Wen Y, She L, Sui YN, Liu L, Richards LJ, Poo MM. Axon position within the corpus callosum determines contralateral cortical projection. Proc Natl Acad Sci U S A 110(29) E2714-23(2013) 23812756 |