Strain Data Sheet

RBRC01104

Strain Information

Image
BRC No.RBRC01104
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Sema3a<tm1.1Tyag>/TyagRbrc
Former Common namesemaphorin III/D (null) Targeting mice
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6NCrlCrlj
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Masahiko Taniguchi and Takeshi Yagi, National Institute for Physiological Sciences in 1997. TT2 ES cells were used. The mutant mice were backcrossed to C57BL/6 over 6 times.
Strain descriptionSemaphorin III/D (Sema3a) knockout mice (null allele). Homozygous mutant mice show decreased body weight. Most of homozygous mice die due to poor growth.
Colony maintenanceHeterozygote x Wild-type [or Crossing to C57BL/6NCrlCrlj]
References
Disruption of semaphorin III/D gene causes severe abnormality in peripheral nerve projection.
Taniguchi M, Yuasa S, Fujisawa H, Naruse I, Saga S, Mishina M, Yagi T
Neuron, 19, 519-530 (1997). 9331345

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Sema3asema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A5Sema3atargeted mutation 1.1, Takeshi Yagi
  • hypogonadotropic hypogonadism 16 with or without anosmia(MedGEN)
    • loxPphage P1 loxP5loxP
  • hypogonadotropic hypogonadism 16 with or without anosmia(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal T cell physiology(MP:0002444)

  • abnormal dendritic cell physiology(MP:0002376)

  • abnormal dorsal root ganglion morphology(MP:0000961)

  • abnormal enteric nervous system morphology(MP:0001044)

  • abnormal olfactory bulb morphology(MP:0000819)
    • more 7 phenotypes
  • abnormal paravertebral ganglion morphology(MP:0008317)

  • abnormal spinal nerve morphology(MP:0001077)

  • abnormal trigeminal nerve morphology(MP:0001065)

  • abnormal vagus nerve morphology(MP:0001074)

  • decreased body size(MP:0001265)

  • decreased body weight(MP:0001262)

  • small olfactory bulb(MP:0002741)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP site, mouse semaphorin 3A genomic DNA
    Research applicationCre/loxP system
    Neurobiology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuron, 19, 519-530 (1997).Any patent rights and other intellectual properties are retained by the depositor. Users have to contact the depositor in the case of application for any patents with the result from these mice.
    When users releases research results that obtained in two years after deposition, users should contact a bailor about collaboration.
    DepositorTakeshi Yagi (National Institute for Physiological Sciences)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-
    Mouse of the Month Aug 2006

    BRC mice in Publications

    Fukuda T, Takeda S, Xu R, Ochi H, Sunamura S, Sato T, Shibata S, Yoshida Y, Gu Z, Kimura A, Ma C, Xu C, Bando W, Fujita K, Shinomiya K, Hirai T, Asou Y, Enomoto M, Okano H, Okawa A, Itoh H.
    Sema3A regulates bone-mass accrual through sensory innervations.
    Nature 497(7450) 490-3(2013) 23644455
    Imai T, Yamazaki T, Kobayakawa R, Kobayakawa K, Abe T, Suzuki M, Sakano H.
    Pre-target axon sorting establishes the neural map topography.
    Science 325(5940) 585-90(2009) 19589963