Strain Data Sheet

RBRC01103

Strain Information

Image
BRC No.RBRC01103
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Camk2a-Fyn-531)1Nko/Rbrc
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Nobuhiko Kojima, National Institute for Physiological Sciences, Japan at 1994. The FynB fragment mutated from TAT to TTT at codon 531 by site-directed mutagenesis under the control of the CaMKII alpha promoter was injected into the pronuclei of B6CBF1 fertilized egges. The transgenic mice were backcross to C57BL/6J mice for 26 generations.Generation:N23+N3
Strain descriptionB6.Cg-Tg(Camk2a-Fyn-531)1Nko/Rbrc. Transgenic mice overexpressing activated form of Fyn under the control of the calcium/calmodulin-dependent protein kinase II alpha promoter. The transgenic mutant Fyn is expressed at high levels in neurons of the forebrain. The transgenic mice exhibit seizure activity by stimulus of drug and light, accelerated kindling, and spontaneous death after weaning. The survival rate of these mice correlates inversely with the expression level of mutant Fyn. This strain is useful for elucidation of the role of Fyn tyrosine kinase in synaptic plasticity.
Colony maintenanceBackcross to C57BL/6 (Hemizygote x C57BL/6JJcl)Reproductive performance is good.
References
Higher seizure susceptibility and enhanced tyrosine phosphorylation of N-methyl-D-aspartate receptor subunit 2B in fyn transgenic mice.
Kojima N, Ishibashi H, Obata K, Kandel E R
Learn. Mem., 5, 429-45 (1998). 10489260

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
FynFyn proto-oncogeneUNFynmouse Camk2a promoter
  • intellectual disability, autosomal dominant 53(MedGEN)

  • intellectual disability, autosomal recessive 63(MedGEN)
  • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal kindling response(MP:0001499)

  • abnormal locomotor behavior(MP:0001392)

  • abnormal motor capabilities/coordination/movement(MP:0002066)

  • abnormal seizure response to electrical stimulation(MP:0001650)

  • alcohol aversion(MP:0002570)
  • more 6 phenotypes
  • decreased anxiety-related response(MP:0001364)

  • decreased behavioral withdrawal response(MP:0009776)

  • impaired behavioral response to alcohol(MP:0009755)

  • increased susceptibility to pharmacologically induced seizures(MP:0002906)

  • premature death(MP:0002083)

  • tonic seizures(MP:0002826)
  • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Fyn B (Y531F) cDNA, mouse CaMK IIα promoter
    Research applicationNeurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Learn. Mem., 5, 429-45 (1998).
    DepositorNobuhiko Kojima (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data