Strain Information | |
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Image | |
BRC No. | RBRC01103 |
Type | Transgene Congenic |
Species | Mus musculus |
Strain name | B6.Cg-Tg(Camk2a-Fyn-531)1Nko/Rbrc |
Former Common name | |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6JJcl |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Dr. Nobuhiko Kojima, National Institute for Physiological Sciences, Japan at 1994. The FynB fragment mutated from TAT to TTT at codon 531 by site-directed mutagenesis under the control of the CaMKII alpha promoter was injected into the pronuclei of B6CBF1 fertilized egges. The transgenic mice were backcross to C57BL/6J mice for 26 generations.Generation:N23+N3 |
Strain description | B6.Cg-Tg(Camk2a-Fyn-531)1Nko/Rbrc. Transgenic mice overexpressing activated form of Fyn under the control of the calcium/calmodulin-dependent protein kinase II alpha promoter. The transgenic mutant Fyn is expressed at high levels in neurons of the forebrain. The transgenic mice exhibit seizure activity by stimulus of drug and light, accelerated kindling, and spontaneous death after weaning. The survival rate of these mice correlates inversely with the expression level of mutant Fyn. This strain is useful for elucidation of the role of Fyn tyrosine kinase in synaptic plasticity. |
Colony maintenance | Backcross to C57BL/6 (Hemizygote x C57BL/6JJcl)Reproductive performance is good. |
References | Higher seizure susceptibility and enhanced tyrosine phosphorylation of N-methyl-D-aspartate receptor subunit 2B in fyn transgenic mice. Kojima N, Ishibashi H, Obata K, Kandel E R Learn. Mem., 5, 429-45 (1998). 10489260 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Fyn | Fyn proto-oncogene | UN | Fyn | mouse Camk2a promoter |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | more 6 phenotypes |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | mouse Fyn B (Y531F) cDNA, mouse CaMK IIα promoter |
Research application | Neurobiology Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Learn. Mem., 5, 429-45 (1998). |
Depositor | Nobuhiko Kojima (RIKEN) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |