Strain Data Sheet

RBRC01101

Strain Information

Image
BRC No.RBRC01101
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129S2-Rbpj<tm1Kyo>/HonRbrc
Former Common nameRBP-J knockout mouse
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strainC57BL/6NCrSlc
Appearanceblack [a/a B/B C/C]
Strain development開発年：1995年開発者：本庶佑先生機関名：京都大学大学院医学研究科D3細胞 (129/Sv系統由来ES細胞）より樹立後、C57BL/6に18代バッククロス済。
Strain descriptionRBP-J遺伝子の古典的ノックアウト
Colony maintenanceヘテロと野生型C57BL/6との交配により維持 (野生型C57BL/6と同時の繁殖効率）。
References
Disruption of the mouse RBP-J kappa gene results in early embryonic death.
C Oka, T Nakano, A Wakeham, J L de la Pompa, C Mori, T Sakai, S Okazaki, M Kawaichi, K Shiota, T W Mak, T Honjo
Development, 121, 3291-3301 (1995). 7588063

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Rbpj
MGI:96522		recombination signal binding protein for immunoglobulin kappa J region5Rbpj<tm1Kyo>
MGI:1857411		targeted mutation 1, Kyoto University
  • Adams-Oliver syndrome 3(MedGEN)
    • neo neomycin resistance gene (E. coli)5 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal anterior cardinal vein morphology (MP:0004784)

  • abnormal aortic valve morphology (MP:0002747)

  • abnormal blood vessel morphology (MP:0001614)

  • abnormal common cardinal vein morphology (MP:0004786)

  • abnormal dorsal aorta morphology (MP:0004787)
    • more 20 phenotypes
  • abnormal fetal cardiomyocyte proliferation (MP:0003567)

  • abnormal heart echocardiography feature (MP:0011925)

  • abnormal humoral immune response (MP:0001800)

  • abnormal myocardium layer morphology (MP:0005329)

  • abnormal placental labyrinth vasculature morphology (MP:0008803)

  • abnormal response to infection (MP:0005025)

  • abnormal vascular regression (MP:0000364)

  • abnormal vitelline vascular remodeling (MP:0004076)

  • aortic valve inflammation (MP:0031124)

  • arteriovenous malformation (MP:0006093)

  • calcified aortic valve cusp (MP:0031518)

  • cardiac fibrosis (MP:0003141)

  • decreased cardiac muscle contractility (MP:0005140)

  • decreased interleukin-4 secretion (MP:0008700)

  • decreased spongiotrophoblast size (MP:0012099)

  • disorganized myocardium (MP:0002190)

  • embryonic growth retardation (MP:0003984)

  • embryonic lethality between somite formation and embryo turning, complete penetrance (MP:0011097)

  • thick aortic valve cusps (MP:0010593)

  • trabecula carnea hypoplasia (MP:0000295)
    • Detailed phenotype data

    Ordering Information

    供与核酸mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse RBP-J genomic DNA
    Research applicationDevelopmental Biology Research
    提供条件1. 公表を前提とした学術研究に限る。
    2. 研究成果の公表にあたって寄託者への謝辞の表明並びに寄託者の指定する文献を引用する。Development, 121, 3291-3301 (1995).
    3. 本件リソースの改変体又は本件リソースの生産方法若しくは使用方法に係る特許を出願した場合、その旨を速やかに寄託者に通知する。
    Depositor本庶　佑（京都大学）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Honjo Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Niwa Y, Masamizu Y, Liu T, Nakayama R, Deng CX, Kageyama R.
    The initiation and propagation of Hes7 oscillation are cooperatively regulated by Fgf and notch signaling in the somite segmentation clock.
    Dev Cell 13(2) 298-304(2007) 17681139