Strain Data Sheet



BRC No.RBRC01101
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129S2-Rbpj<tm1Kyo>/HonRbrc
Former Common nameRBP-J knockout mouse
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strainC57BL/6NCrSlc
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Honjo, Faculty of Medicine, Kyoto University in 1995.
Strain descriptionRBP-J, recombining binding protein suppressor of hairless (Drosophila) which is a transcriptional factor of a Notch/RBP-J signaling pathway play a role in fate determination in various lineages. RBP-J homozygous mutant mice show severe growth retardation at 8.5 days of gestation, incomplete turning of the body axis, microencephaly, abnormal placental development, anterior neuropore opening and defective somitogenesis at 9.5 days of gestation, and die before 10.5 days of gestation.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6CrSlc)
ReferencesDevelopment, 121, 3291-3301 (1995). 7588063

Health Report

Examination Date / Room / Racknull


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Rbpjrecombining binding protein suppressor of hairless (Drosophila)5Rbpj<tm1Kyo>targeted mutation 1, Kyoto UniversityRBPjk-, RBP-Jkappa-

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)5neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)


Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse RBP-J genomic DNA
Research applicationDevelopmental Biology Research
Specific Term and Conditions1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Development, 121, 3291-3301 (1995).
3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
DepositorTasuku Honjo (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Honjo Lab HP

BRC mice in Publications

Niwa Y, Masamizu Y, Liu T, Nakayama R, Deng CX, Kageyama R.
The initiation and propagation of Hes7 oscillation are cooperatively regulated by Fgf and notch signaling in the somite segmentation clock.
Dev Cell 13(2) 298-304(2007) 17681139