Strain Data Sheet

RBRC01099

update : 2024-04-19

Strain Information
Image
BRC No.	RBRC01099
Type	Transgene
Species	Mus musculus
Strain name	C57BL/6J-Tg(CAG-XBP1*/venus)#Miur/MiurRbrc
Former Common name	ER stress-activated indicator (ERAI), ERAI mice
H-2 Haplotype
ES Cell line
Background strain	C57BL/6JJcl
Appearance	black [a/a B/B C/C]
Strain development	開発年：2002年開発者：岩脇隆夫先生、三浦正幸先生機関名：理研BSI
Strain description	ヒトXBP1遺伝子とvenus (改良型GFP) 遺伝子との融合遺伝子をトランスジーンとして作製したトランスジェニックマウスで、小胞体ストレスを受けた組織は蛍光を発する。
Colony maintenance	ヘテロ型である場合は野生型C57BL/6と同様に飼育・交配が可能である。ホモ型の表現型は未確認。
References	Nat. Med., 10, 98-102 (2004). 14702639

Health Report
Examination Date / Room / Rack	2024/02/19Room:4-ARack:C 2023/11/20Room:4-ARack:C 2023/11/13Room:4-ARack:C 2023/08/21Room:4-ARack:C 2023/08/07Room:4-ARack:C 2023/05/22Room:4-ARack:C 2023/02/20Room:4-ARack:C 2022/11/21Room:4-ARack:C 2022/08/22Room:4-ARack:C 2022/05/23Room:4-ARack:C

Gene
Gene info	Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter FLAGFLAG tag (synthetic)UNFLAGCMV-IE enhancer, chicken beta-actin promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter VenusYellow Fluorescent Protein (Aequorea victoria)UNVenusVenus Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter XBP1X-box binding protein 1 (human)UNXBP1D11Ertd39e, TREB-5, TREB5, TREB5, XBP-1

Gene

Gene info

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter

FLAGFLAG tag (synthetic)UNFLAGCMV-IE enhancer, chicken beta-actin promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter

VenusYellow Fluorescent Protein (Aequorea victoria)UNVenusVenus

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter

XBP1X-box binding protein 1 (human)UNXBP1D11Ertd39e, TREB-5, TREB5, TREB5, XBP-1

Ordering Information
供与核酸	CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), SV40 intron and polyA signal, human XBP1 cDNA, Jellyfish venus (GFP) cDNA, FLAG tag
Research application	Cell Biology Research Fluorescent Proteins/lacZ System
提供条件	条件を付加する。利用者は事前に寄託者の提供承諾書を得る。研究成果の公表にあたって寄託者の指定する文献を引用する。Nat. Med., 10, 98-102 (2004). 研究成果の公表にあたって謝辞の表明を必要とする。寄託後２年間は共同研究として用い、論文や学会での発表の際は共著とする。寄託者の研究と競合する用途を避けるために、予定の研究概要を寄託者に知らせ、使用許可を得る。利用は非商業的研究・教育目的とする。もし商業的利用をする場合は理化学研究所と協議する。本試料は理化学研究所の成果であることを明記する。研究成果の公表にあたってVenusの開発者である宮脇敦史先生への謝辞の表明並びに指定する文献を引用する。Nat. Biotechnol., 20, 87-90 (2002).
Depositor	三浦　正幸（国立大学法人東京大学）
Strain Status	生体凍結胚凍結精子
Strain Availability	凍結精子を1ヶ月以内に提供可能凍結胚を1ヶ月以内に提供可能生体マウスを1~3ヶ月以内に提供可能
Additional Info.	Necessary documents for ordering: Approval form (Japanese / English) Order form (Japanese / English) Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English) CAGGS MTA (English) Acceptance of responsibility for living modified organism (Japanese / English) Lab HP (Japanese) Genotyping protocol -PCR- Genetic Background Mouse of the Month Mar 2007

BRC mice in Publications

Norlin S, Parekh VS, Naredi P, Edlund H. Asna1/TRC40 Controls β-Cell Function and Endoplasmic Reticulum Homeostasis by Ensuring Retrograde Transport. Diabetes 65(1) 110-9(2016) 26438609 Wang X, Wang G, Kunte M, Shinde V, Gorbatyuk M. Modulation of angiogenesis by genetic manipulation of ATF4 in mouse model of oxygen-induced retinopathy [corrected]. Invest Ophthalmol Vis Sci 54(9) 5995-6002(2013) 23942974 Kunte MM, Choudhury S, Manheim JF, Shinde VM, Miura M, Chiodo VA, Hauswirth WW, Gorbatyuk OS, Gorbatyuk MS. ER stress is involved in T17M rhodopsin-induced retinal degeneration. Invest Ophthalmol Vis Sci 53(7) 3792-800(2012) 22589437 Martino ME, Olsen JC, Fulcher NB, Wolfgang MC, O'Neal WK, Ribeiro CM. Airway epithelial inflammation-induced endoplasmic reticulum Ca2+ store expansion is mediated by X-box binding protein-1. J Biol Chem 284(22) 14904-13(2009) 19321437 Kamimura D, Bevan MJ. Endoplasmic reticulum stress regulator XBP-1 contributes to effector CD8+ T cell differentiation during acute infection. J Immunol 181(8) 5433-41(2008) 18832700 Bhootada Y, Kotla P, Zolotukhin S, Gorbatyuk O, Bebok Z, Athar M, Gorbatyuk M. Limited ATF4 Expression in Degenerating Retinas with Ongoing ER Stress Promotes Photoreceptor Survival in a Mouse Model of Autosomal Dominant Retinitis Pigmentosa. PLoS One 11(5) e0154779(2016) 27144303 Cao L, Kobayakawa S, Yoshiki A, Abe K. High resolution intravital imaging of subcellular structures of mouse abdominal organs using a microstage device. PLoS One 7(3) e33876(2012) 22479464 Braun, M., Tatreau, J., Fulcher, N., Wolfgang, M., Randell, S., O’Neal, W., and Ribeiro, C. M. P. Pseudomonas aeruginosa activates the unfolded protein response in airway epithelia: relevance for airway ・ inflammation. Pediatric Pulmonology, Supplement 30 260(2007)

BRC mice in Publications

Norlin S, Parekh VS, Naredi P, Edlund H.
Asna1/TRC40 Controls β-Cell Function and Endoplasmic Reticulum Homeostasis by Ensuring Retrograde Transport.
Diabetes 65(1) 110-9(2016) 26438609

Wang X, Wang G, Kunte M, Shinde V, Gorbatyuk M.
Modulation of angiogenesis by genetic manipulation of ATF4 in mouse model of oxygen-induced retinopathy [corrected].
Invest Ophthalmol Vis Sci 54(9) 5995-6002(2013) 23942974

Kunte MM, Choudhury S, Manheim JF, Shinde VM, Miura M, Chiodo VA, Hauswirth WW, Gorbatyuk OS, Gorbatyuk MS.
ER stress is involved in T17M rhodopsin-induced retinal degeneration.
Invest Ophthalmol Vis Sci 53(7) 3792-800(2012) 22589437

Martino ME, Olsen JC, Fulcher NB, Wolfgang MC, O'Neal WK, Ribeiro CM.
Airway epithelial inflammation-induced endoplasmic reticulum Ca2+ store expansion is mediated by X-box binding protein-1.
J Biol Chem 284(22) 14904-13(2009) 19321437

Kamimura D, Bevan MJ.
Endoplasmic reticulum stress regulator XBP-1 contributes to effector CD8+ T cell differentiation during acute infection.
J Immunol 181(8) 5433-41(2008) 18832700

Bhootada Y, Kotla P, Zolotukhin S, Gorbatyuk O, Bebok Z, Athar M, Gorbatyuk M.
Limited ATF4 Expression in Degenerating Retinas with Ongoing ER Stress Promotes Photoreceptor Survival in a Mouse Model of Autosomal Dominant Retinitis Pigmentosa.
PLoS One 11(5) e0154779(2016) 27144303

Cao L, Kobayakawa S, Yoshiki A, Abe K.
High resolution intravital imaging of subcellular structures of mouse abdominal organs using a microstage device.
PLoS One 7(3) e33876(2012) 22479464

Braun, M., Tatreau, J., Fulcher, N., Wolfgang, M., Randell, S., O’Neal, W., and Ribeiro, C. M. P.
Pseudomonas aeruginosa activates the unfolded protein response in airway epithelia: relevance for airway ・ inflammation.
Pediatric Pulmonology, Supplement 30 260(2007)

Strain Information

Health Report

Gene

Ordering Information

BRC mice in Publications