Strain Data Sheet

RBRC01071

Strain Information

Image
BRC No.RBRC01071
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Rbpj<tm1Hon>/HonRbrc
Former Common nameFloxed RBP-J
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Tasuku Honjo, Graduate School of Medicine, Kyoto University. The construct was electoroporated into E14 ES cells derived from 129P2/OlaHsd. The mutant mice were crossed to C57BL/6.
Strain descriptionRbpj floxed mice. Rbpj, recombination signal binding protein for immunoglobulin kappa J region is a transcriptional factor of a Notch/RBP-J signaling pathway play a role in fate determination in various lineages. Exon 6 and 7 of Rbpj gene was flanked by loxP sites, and a neomycin resistance gene was inserted between exon 7 and the loxP site. Conditional RBP-J deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenanceHomozygote x Homozygote. Homozygous mice are viable and fertile.
ReferencesInt. Immunol., 14, 637-645 (2002). 12039915

Health Report

Examination Date / Room / Rack2024/02/26Room:4-CRack:C
2023/12/04Room:4-CRack:C
2023/11/27Room:4-CRack:C
2023/08/28Room:4-CRack:C
2023/05/29Room:4-CRack:C
2023/02/27Room:4-CRack:C
2022/11/29Room:4-CRack:C
2022/08/29Room:4-CRack:C
2022/05/31Room:4-CRack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Rbpjrecombining binding protein suppressor of hairless (Drosophila)5Rbpj<tm1Hon>targeted mutation 1, Tasuku HonjoRBPf, RBP-JC, RBP-Jf, Rbpjflox, Rbp-Jflox, RBP-JkappaFl, RBP-Jkappaflox, RBPJkappaloxP, RBP-Jlox, RbpjloxP, Rbpsuhtm3Kyo

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)5neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

供与核酸phage P1 loxP sites, E. coli neo, mouse RBP-J genomic DNA, mouse phosphoglycerate kinase promoter (PGK promoter)
Research applicationCre/loxP system
提供条件条件を付加する。
1. 公表を前提とした学術研究に限る。
2. 研究成果の公表にあたって寄託者への謝辞の表明並びに寄託者の指定する文献を引用する。Int, Immunol., 14, 637-645 (2002).
3. 本件リソースの改変体又は本件リソースの生産方法若しくは使用方法に係る特許を出願した場合、その旨を速やかに寄託者に通知する。
Depositor本庶　佑（京都大学）
Strain Status生体のアイコン生体
凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
生体マウスを1~3ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Honjo Lab HP
Genotyping protocol -PCR-
Genetic Background

BRC mice in Publications

Fujimoto M, Takagi Y, Muraki K, Nozaki K, Yamamoto N, Tsuji M, Hashimoto N, Honjo T, Tanigaki K.
RBP-J promotes neuronal differentiation and inhibits oligodendroglial development in adult neurogenesis.
Dev Biol 332(2) 339-50(2009) 19501584

Niwa Y, Masamizu Y, Liu T, Nakayama R, Deng CX, Kageyama R.
The initiation and propagation of Hes7 oscillation are cooperatively regulated by Fgf and notch signaling in the somite segmentation clock.
Dev Cell 13(2) 298-304(2007) 17681139

Liu Z, Owen T, Fang J, Srinivasan RS, Zuo J.
In vivo Notch reactivation in differentiating cochlear hair cells induces Sox2 and Prox1 expression but does not disrupt hair cell maturation.
Dev Dyn 241(4) 684-96(2012) 22354878

Mead TJ, Yutzey KE.
Notch pathway regulation of neural crest cell development in vivo.
Dev Dyn 241(2) 376-89(2012) 22275227

Zong Y, Panikkar A, Xu J, Antoniou A, Raynaud P, Lemaigre F, Stanger BZ.
Notch signaling controls liver development by regulating biliary differentiation.
Development 136(10) 1727-39(2009) 19369401

Obata Y, Kimura S, Nakato G, Iizuka K, Miyagawa Y, Nakamura Y, Furusawa Y, Sugiyama M, Suzuki K, Ebisawa M, Fujimura Y, Yoshida H, Iwanaga T, Hase K, Ohno H.
Epithelial-stromal interaction via Notch signaling is essential for the full maturation of gut-associated lymphoid tissues.
EMBO Rep 15(12) 1297-304(2014) 25378482

Sawaguchi S, Varshney S, Ogawa M, Sakaidani Y, Yagi H, Takeshita K, Murohara T, Kato K, Sundaram S, Stanley P, Okajima T.
O-GlcNAc on NOTCH1 EGF repeats regulates ligand-induced Notch signaling and vascular development in mammals.
Elife 6 (2017) 28395734

Nakamura M, Shibata K, Hatano S, Sato T, Ohkawa Y, Yamada H, Ikuta K, Yoshikai Y.
A genome-wide analysis identifies a notch-RBP-Jκ-IL-7Rα axis that controls IL-17-producing γδ T cell homeostasis in mice.
J Immunol 194(1) 243-51(2015) 25429074

Obata Y, Takahashi D, Ebisawa M, Kakiguchi K, Yonemura S, Jinnohara T, Kanaya T, Fujimura Y, Ohmae M, Hase K, Ohno H.
Epithelial cell-intrinsic Notch signaling plays an essential role in the maintenance of gut immune homeostasis.
J Immunol 188(5) 2427-36(2012) 22279105

Ito T, Kwon HY, Zimdahl B, Congdon KL, Blum J, Lento WE, Zhao C, Lagoo A, Gerrard G, Foroni L, Goldman J, Goh H, Kim SH, Kim DW, Chuah C, Oehler VG, Radich JP, Jordan CT, Reya T.
Regulation of myeloid leukaemia by the cell-fate determinant Musashi.
Nature 466(7307) 765-8(2010) 20639863

Hosaka Y, Saito T, Sugita S, Hikata T, Kobayashi H, Fukai A, Taniguchi Y, Hirata M, Akiyama H, Chung UI, Kawaguchi H.
Notch signaling in chondrocytes modulates endochondral ossification and osteoarthritis development.
Proc Natl Acad Sci U S A 110(5) 1875-80(2013) 23319657