Strain Data Sheet

RBRC01071

Strain Information

Image
BRC No.RBRC01071
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Rbpj<tm1Hon>/HonRbrc
Former Common nameFloxed RBP-J
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Tasuku Honjo, Graduate School of Medicine, Kyoto University. The construct was electoroporated into E14 ES cells derived from 129P2/OlaHsd. The mutant mice were crossed to C57BL/6.
Strain descriptionRbpj floxed mice. Rbpj, recombination signal binding protein for immunoglobulin kappa J region is a transcriptional factor of a Notch/RBP-J signaling pathway play a role in fate determination in various lineages. Exon 6 and 7 of Rbpj gene was flanked by loxP sites, and a neomycin resistance gene was inserted between exon 7 and the loxP site. Conditional RBP-J deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenanceHomozygote x Homozygote. Homozygous mice are viable and fertile.
References
Inducible gene knockout of transcription factor recombination signal binding protein-J reveals its essential role in T versus B lineage decision.
Han H, Tanigaki K, Yamamoto N, Kuroda K, Yoshimoto M, Nakahata T, Ikuta K, Honjo T
Int. Immunol., 14, 637-645 (2002). 12039915

Health Report

Examination Date / Room / Rack2024/05/27Room:4-CRack:C
2024/02/26Room:4-CRack:C
2023/12/04Room:4-CRack:C
2023/11/27Room:4-CRack:C
2023/08/28Room:4-CRack:C
2023/05/29Room:4-CRack:C
2023/02/27Room:4-CRack:C
2022/11/29Room:4-CRack:C

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Rbpjrecombination signal binding protein for immunoglobulin kappa J region5Rbpjtargeted mutation 1, Tasuku Honjo
  • Adams-Oliver syndrome 3(MedGEN)
    • loxPphage P1 loxP5loxP
  • Adams-Oliver syndrome 3(MedGEN)
    • loxPphage P1 loxP5loxP
  • Adams-Oliver syndrome 3(MedGEN)
    • neoneomycin resistance gene (E. coli)5mouse phosphoglycerate kinase promoter (PGK promoter)
  • Adams-Oliver syndrome 3(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal B cell differentiation(MP:0002144)

  • abnormal CD4-positive helper T cell morphology(MP:0010183)

  • abnormal T-helper 2 cell differentiation(MP:0008092)

  • abnormal anterior cardinal vein morphology(MP:0004784)

  • abnormal atrioventricular cushion morphology(MP:0000297)
    • more 73 phenotypes
  • abnormal cardiac epithelial to mesenchymal transition(MP:0008825)

  • abnormal cell differentiation(MP:0005076)

  • abnormal centroacinar cell of Langerhans morphology(MP:0010171)

  • abnormal common cardinal vein morphology(MP:0004786)

  • abnormal crypts of Lieberkuhn morphology(MP:0000490)

  • abnormal cytokine secretion(MP:0003009)

  • abnormal diaphragm morphology(MP:0002279)

  • abnormal epaxial muscle morphology(MP:0004248)

  • abnormal glutaminergic neuron morphology(MP:0003247)

  • abnormal heart development(MP:0000267)

  • abnormal immune system physiology(MP:0001790)

  • abnormal intercostal muscle morphology(MP:0002280)

  • abnormal intestinal epithelium morphology(MP:0000488)

  • abnormal intestinal goblet cell morphology(MP:0003449)

  • abnormal lymphopoiesis(MP:0002401)

  • abnormal mammary gland growth during pregnancy(MP:0006269)

  • abnormal myogenesis(MP:0000729)

  • abnormal neural tube morphology(MP:0002151)

  • abnormal neuron differentiation(MP:0009937)

  • abnormal pancreas physiology(MP:0002693)

  • abnormal proximal convoluted tubule morphology(MP:0004756)

  • abnormal regulatory T cell physiology(MP:0004946)

  • abnormal respiratory epithelium morphology(MP:0010942)

  • abnormal spinal cord interneuron morphology(MP:0004100)

  • abnormal suckling behavior(MP:0001436)

  • abnormal vascular regression(MP:0000364)

  • absent club cells(MP:0011071)

  • absent podocytes(MP:0008137)

  • absent vitelline blood vessels(MP:0001719)

  • arrested T cell differentiation(MP:0001825)

  • arteriovenous malformation(MP:0006093)

  • decreased dendritic cell number(MP:0008127)

  • decreased double-positive T cell number(MP:0005092)

  • decreased follicular dendritic cell number(MP:0008200)

  • decreased marginal zone B cell number(MP:0008182)

  • decreased neuronal precursor cell number(MP:0004981)

  • decreased satellite cell number(MP:0003833)

  • decreased single-positive T cell number(MP:0008083)

  • decreased skeletal muscle fiber density(MP:0009408)

  • decreased thymocyte number(MP:0000715)

  • decreased tumor growth/size(MP:0003447)

  • diluted coat color(MP:0000371)

  • embryonic growth retardation(MP:0003984)

  • embryonic lethality during organogenesis, complete penetrance(MP:0011098)

  • embryonic lethality, incomplete penetrance(MP:0011102)

  • epidermal cyst(MP:0003414)

  • hypaxial muscle hypoplasia(MP:0003359)

  • hyperkeratosis(MP:0001242)

  • immune system phenotype(MP:0005387)

  • increased B cell number(MP:0005014)

  • increased IgG3 level(MP:0008502)

  • increased T cell number(MP:0005015)

  • increased T cell proliferation(MP:0005348)

  • increased double-negative T cell number(MP:0005090)

  • increased follicular B cell number(MP:0008173)

  • increased macrophage cell number(MP:0005425)

  • increased monocyte cell number(MP:0000220)

  • increased pancreatic acinar cell number(MP:0009159)

  • increased plasmacytoid dendritic cell number(MP:0008524)

  • increased single-positive T cell number(MP:0008082)

  • increased splenocyte number(MP:0009338)

  • increased susceptibility to bacterial infection induced morbidity/mortality(MP:0009788)

  • increased susceptibility to bacterial infection(MP:0002412)

  • loss of GABAergic neurons(MP:0003246)

  • loss of vibrissae(MP:0001280)

  • neonatal lethality, complete penetrance(MP:0011087)

  • no spontaneous movement(MP:0001404)

  • pericardial effusion(MP:0005312)

  • pigmentation phenotype(MP:0001186)

  • premature hair loss(MP:0005114)

  • respiratory failure(MP:0001953)

  • small thymus(MP:0000706)

  • thick epidermis(MP:0001219)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, E. coli neo, mouse RBP-J genomic DNA, mouse phosphoglycerate kinase promoter (PGK promoter)
    Research applicationCre/loxP system
    Specific Term and Conditions1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
    2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Int, Immunol., 14, 637-645 (2002).
    3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
    DepositorTasuku Honjo (Kyoto University)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Honjo Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Fujimoto M, Takagi Y, Muraki K, Nozaki K, Yamamoto N, Tsuji M, Hashimoto N, Honjo T, Tanigaki K.
    RBP-J promotes neuronal differentiation and inhibits oligodendroglial development in adult neurogenesis.
    Dev Biol 332(2) 339-50(2009) 19501584
    Niwa Y, Masamizu Y, Liu T, Nakayama R, Deng CX, Kageyama R.
    The initiation and propagation of Hes7 oscillation are cooperatively regulated by Fgf and notch signaling in the somite segmentation clock.
    Dev Cell 13(2) 298-304(2007) 17681139
    Liu Z, Owen T, Fang J, Srinivasan RS, Zuo J.
    In vivo Notch reactivation in differentiating cochlear hair cells induces Sox2 and Prox1 expression but does not disrupt hair cell maturation.
    Dev Dyn 241(4) 684-96(2012) 22354878
    Mead TJ, Yutzey KE.
    Notch pathway regulation of neural crest cell development in vivo.
    Dev Dyn 241(2) 376-89(2012) 22275227
    Zong Y, Panikkar A, Xu J, Antoniou A, Raynaud P, Lemaigre F, Stanger BZ.
    Notch signaling controls liver development by regulating biliary differentiation.
    Development 136(10) 1727-39(2009) 19369401
    Obata Y, Kimura S, Nakato G, Iizuka K, Miyagawa Y, Nakamura Y, Furusawa Y, Sugiyama M, Suzuki K, Ebisawa M, Fujimura Y, Yoshida H, Iwanaga T, Hase K, Ohno H.
    Epithelial-stromal interaction via Notch signaling is essential for the full maturation of gut-associated lymphoid tissues.
    EMBO Rep 15(12) 1297-304(2014) 25378482
    Sawaguchi S, Varshney S, Ogawa M, Sakaidani Y, Yagi H, Takeshita K, Murohara T, Kato K, Sundaram S, Stanley P, Okajima T.
    O-GlcNAc on NOTCH1 EGF repeats regulates ligand-induced Notch signaling and vascular development in mammals.
    Elife 6 (2017) 28395734
    Nakamura M, Shibata K, Hatano S, Sato T, Ohkawa Y, Yamada H, Ikuta K, Yoshikai Y.
    A genome-wide analysis identifies a notch-RBP-Jκ-IL-7Rα axis that controls IL-17-producing γδ T cell homeostasis in mice.
    J Immunol 194(1) 243-51(2015) 25429074
    Obata Y, Takahashi D, Ebisawa M, Kakiguchi K, Yonemura S, Jinnohara T, Kanaya T, Fujimura Y, Ohmae M, Hase K, Ohno H.
    Epithelial cell-intrinsic Notch signaling plays an essential role in the maintenance of gut immune homeostasis.
    J Immunol 188(5) 2427-36(2012) 22279105
    Ito T, Kwon HY, Zimdahl B, Congdon KL, Blum J, Lento WE, Zhao C, Lagoo A, Gerrard G, Foroni L, Goldman J, Goh H, Kim SH, Kim DW, Chuah C, Oehler VG, Radich JP, Jordan CT, Reya T.
    Regulation of myeloid leukaemia by the cell-fate determinant Musashi.
    Nature 466(7307) 765-8(2010) 20639863
    Hosaka Y, Saito T, Sugita S, Hikata T, Kobayashi H, Fukai A, Taniguchi Y, Hirata M, Akiyama H, Chung UI, Kawaguchi H.
    Notch signaling in chondrocytes modulates endochondral ossification and osteoarthritis development.
    Proc Natl Acad Sci U S A 110(5) 1875-80(2013) 23319657