Strain Information | |
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Image | |
BRC No. | RBRC01058 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | ICR.Cg-Nrp1<tm1Hfu>/HfuRbrc |
Former Common name | ICR-Nrp1<tmHfu> |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | ICR |
Appearance | albino |
Strain development | Developed by Dr. Hajime Fujisawa, Nagoya University in 1997. A neomycin selection cassette was inserted into a fragment of the sequence encoding the a1 domain of the protein. ICR background. |
Strain description | ICR.Cg-Nrp1<tm1Hfu>. Neuropilin-1 (Nrp1) is a type I membrane protein and has been shown to function as a receptor for semaphorin III/D. Nrp1 homozygous mutant mice were embryonic lethal, and show severe abnormalities in the trajectory of the cranial and spinal efferent fibers of the parasympathetic nerve system. |
Colony maintenance | Sibling Mating (Heterozygote x Wild-type, Heterozygote) or Cross to ICR (Heterozygote x Slc:ICR)Embryonic lethality (Homozygous). Homozygote mutant mice die up to E11. |
References | Neuropilin-semaphorin III/D-mediated chemorepulsive signals play a crucial role in peripheral nerve projection in mice. Kitsukawa T, Shimizu M, Sanbo M, Hirata T, Taniguchi M, Bekku Y, Yagi T, Fujisawa H Neuron, 19, 995-1005 (1997). 9390514 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Nrp1 | neuropilin 1 | 8 | Nrp1 | targeted mutation 1, Hajime Fujisawa | |||
neo | neomycin resistance gene (E. coli) | 8 | herpes simplex virus thymidine kinase promoter (HSV tk promoter) |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | more 20 phenotypes |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, SV40 poly A, mouse C57BL/6 Neuropilin-1 genomic DNA |
Research application | Cardiovascular Research Developmental Biology Research Hematological Research Immunology and Inflammation Research Neurobiology Research covid19_infection |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuron, 19, 995-1005 (1997). |
Depositor | Hajime Fujisawa (Nagoya University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Hatanaka Y, Matsumoto T, Yanagawa Y, Fujisawa H, Murakami F, Masu M. Distinct roles of neuropilin 1 signaling for radial and tangential extension of callosal axons. J Comp Neurol 514(3) 215-25(2009) 19296474 |