Strain Data Sheet

RBRC01057

Information1

Image
BRC No.RBRC01057
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Nrp1<tmHfu>
Former Common nameC57BL/6-Nrp1<tmHfu>
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6NCrSlc
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Hajime Fujisawa, Nagoya University in 1997. A neomycin selection cassette was inserted into a fragment of the sequence encoding the a1 domain of the protein.C57BL/6 background.
Strain descriptionB6.Cg-Nrp1<tm1Hfu>. Neuropilin-1, Nrp1 is a type I membrane protein and has been shown to function as a receptor for semaphorin III/D.Nrp1 homozygous mutant mice were embryonic lethal, and show severe abnormalities in the trajectory of the cranial and spinal efferent fibers of the parasympathetic nerve system.
Colony maintenanceSibling Mating (Heterozygote x Wild, Heterozygote) Embryonic lethality (Homozygous). Homozygote mutant mice die up to E11.
ReferencesNeuron, 19, 995-1005 (1997). 9390514

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Nrp1neuropilin8Nrp1<tm1Hfu>targeted mutation 1, Hajime FujisawaNeuropilin-1, NP-1, NPN-1, Npn-1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)8neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, SV40 poly A, mouse C57BL/6 Neuropilin-1 genomic DNA
Research applicationCardiovascular Research
Developmental Biology Research
Hematological Research
Immunology and Inflammation Research
Neurobiology Research
covid19_infection
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuron, 19, 995-1005 (1997).
DepositorHajime Fujisawa (Nagoya University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data