Strain Data Sheet

RBRC01057

Strain Information

Image
BRC No.RBRC01057
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Nrp1<tmHfu>
Former Common nameC57BL/6-Nrp1<tmHfu>
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6NCrSlc
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Hajime Fujisawa, Nagoya University in 1997. A neomycin selection cassette was inserted into a fragment of the sequence encoding the a1 domain of the protein.C57BL/6 background.
Strain descriptionB6.Cg-Nrp1<tm1Hfu>. Neuropilin-1, Nrp1 is a type I membrane protein and has been shown to function as a receptor for semaphorin III/D.Nrp1 homozygous mutant mice were embryonic lethal, and show severe abnormalities in the trajectory of the cranial and spinal efferent fibers of the parasympathetic nerve system.
Colony maintenanceSibling Mating (Heterozygote x Wild, Heterozygote) Embryonic lethality (Homozygous). Homozygote mutant mice die up to E11.
References
Neuropilin-semaphorin III/D-mediated chemorepulsive signals play a crucial role in peripheral nerve projection in mice.
Kitsukawa T, Shimizu M, Sanbo M, Hirata T, Taniguchi M, Bekku Y, Yagi T, Fujisawa H
Neuron, 19, 995-1005 (1997). 9390514

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Nrp1neuropilin 18Nrp1targeted mutation 1, Hajime Fujisawa
neoneomycin resistance gene (E. coli)8herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal aortic arch and aortic arch branch attachment(MP:0010464)

  • abnormal axon guidance(MP:0002961)

  • abnormal brain vasculature morphology(MP:0004950)

  • abnormal dorsal aorta morphology(MP:0004787)

  • abnormal facial motor nucleus morphology(MP:0000909)
  • more 22 phenotypes
  • abnormal facial nerve morphology(MP:0001071)

  • abnormal first pharyngeal arch artery morphology(MP:0010355)

  • abnormal fourth pharyngeal arch artery morphology(MP:0006354)

  • abnormal neuronal migration(MP:0006009)

  • abnormal pharyngeal arch artery morphology(MP:0002672)

  • abnormal pulmonary artery morphology(MP:0000484)

  • abnormal second pharyngeal arch artery morphology(MP:0010356)

  • abnormal sixth pharyngeal arch artery morphology(MP:0006355)

  • abnormal spinal nerve morphology(MP:0001077)

  • abnormal third pharyngeal arch artery morphology(MP:0006356)

  • abnormal trigeminal nerve morphology(MP:0001065)

  • abnormal vascular development(MP:0000259)

  • decreased angiogenesis(MP:0005602)

  • decreased neuron number(MP:0008948)

  • disorganized yolk sac vascular plexus(MP:0001723)

  • double aortic arch(MP:0004159)

  • embryonic lethality during organogenesis, complete penetrance(MP:0011098)

  • embryonic lethality, complete penetrance(MP:0011092)

  • nervous system phenotype(MP:0003631)

  • persistent truncus arteriosus(MP:0002633)

  • right aortic arch(MP:0004158)

  • transposition of great arteries(MP:0004110)
  • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, SV40 poly A, mouse C57BL/6 Neuropilin-1 genomic DNA
    Research applicationCardiovascular Research
    Developmental Biology Research
    Hematological Research
    Immunology and Inflammation Research
    Neurobiology Research
    covid19_infection
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuron, 19, 995-1005 (1997).
    DepositorHajime Fujisawa (Nagoya University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data