Strain Data Sheet

RBRC01047

Strain Information

Image
BRC No.RBRC01047
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Creb5<tm1Sis>/Rbrc
Former Common nameCRE-BPa Knock out
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Drs. Toshio Maekawa and Shunsuke Ishii, RIKEN Tsukuba Institute in 1999.
Strain descriptionCRE-Bpa (Creb5) knockout mice. Creb5 gene is a transcription factor including a putative metal finger structure and a DNA-binding domain consisting of a basic amino acid cluster and a leucine zipper. Homozygous mutant mice are postnatal lethal.
Colony maintenance
References
The role of ATF-2 family transcription factors in adipocyte differentiation: antiobesity effects of p38 inhibitors.
Maekawa T, Jin W, Ishii S
Mol. Cell Biol., 30, 613-625 (2010). 19948881

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Creb5cAMP responsive element binding protein 56Creb5targeted mutation 1, Shunsuke Ishii
neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • neonatal lethality, complete penetrance(MP:0011087)
  • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Creb5 genomic DNA
    Research applicationCell Biology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell Biol., 30, 613-625 (2010).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorShunsuke Ishii (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data