Strain Data Sheet
RBRC00980
Strain Information | |
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| Image | |
| BRC No. | RBRC00980 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | B6C3F1-Tg(CAG-EGFP)CX-FM038Osb |
| Former Common name | 152A; B6C3F1B6C3F1-TgN(act-EGFP)OsbCX-FM038 |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | Slc:B6C3F1 |
| Appearance | black or agouti [a/a B/B C/C or A/? B/B C/C] |
| Strain development | Developed by Dr. Masaru Okabe at Osaka University in 1997. The CAG-GFP plasmids were injected into the pronuclei of B6C3F2 fertilized eggs. |
| Strain description | The CAG-EGFP transgene is located on X chromosome. This strain is useful for detection of cell fate in transplantation and developmental studies. |
| Colony maintenance | Sibling Mating |
| References | 'Green mice' as a source of ubiquitous green cells. M Okabe, M Ikawa, K Kominami, T Nakanishi, Y Nishimune FEBS Lett., 407, 313-319 (1997). 9175875FISH analysis of 142 EGFP transgene integration sites into the mouse genome. Tomoko Nakanishi, Asato Kuroiwa, Shuichi Yamada, Ayako Isotani, Atsuko Yamashita, Ai Tairaka, Tamon Hayashi, Tatsuya Takagi, Masahito Ikawa, Yoichi Matsuda, Masaru Okabe Genomics, 80, 564-574 (2002). 12504848Comparison of gene expression in male and female mouse blastocysts revealed imprinting of the X-linked gene, Rhox5/Pem, at preimplantation stages. Shin Kobayashi, Ayako Isotani, Nathan Mise, Masamichi Yamamoto, Yoshitaka Fujihara, Kazuhiro Kaseda, Tomoko Nakanishi, Masahito Ikawa, Hiroshi Hamada, Kuniya Abe, Masaru Okabe Curr. Biol., 16(2):166-172 (2006). 16431368 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| EGFP | Green Fluorescent Protein (Aequorea victoria) | X | EGFP | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | |||
Phenotype | |
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| Phenotype annotation from literatures by Mammalian phenotype ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Jellyfish GFP cDNA |
| Research application | Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genomics, 80, 564-574 (2002). Curr. Biol., 16(2):166-172 (2006). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
| Depositor | Masaru Okabe (Osaka University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Watanabe-Takano H, Takano K, Sakamoto A, Matsumoto K, Tokuhisa T, Endo T, Hatano M. DA-Raf-dependent inhibition of the Ras-ERK signaling pathway in type 2 alveolar epithelial cells controls alveolar formation. Proc Natl Acad Sci U S A 111(22) E2291-300(2014) 24843139 |
Kobayashi S, Fujihara Y, Mise N, Kaseda K, Abe K, Ishino F, Okabe M. The X-linked imprinted gene family Fthl17 shows predominantly female expression following the two-cell stage in mouse embryos. Nucleic Acids Res 38(11) 3672-81(2010) 20185572 |