Strain Data Sheet

RBRC00905

Strain Information

Image
BRC No.RBRC00905
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(dLck-GFP)1Naka/Rbrc
Former Common nameC57BL/6J-Tg(dLck-EGFP), dlck-GFP Tg
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack
Strain developmentDeveloped by Nakayama T, Dept. Mol. Immunol., Graduate Sch. Med., Chiba Univ.
Strain descriptionThis transgenic mice express green fluorescence protein (GFP) under the control of the distal promotor of Lck.
Colony maintenanceHemizygotes x C57BL/6. Transgenic mice may breed as well as wild-type mice.
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFPGreen Fluorescent Protein (Aequorea victoria)UNlck distal promoter, human growth hormone enhancer
  • severe combined immunodeficiency due to LCK deficiency(MedGEN)
    • GHGrowth hormone polyA (human)UNGH
  • severe combined immunodeficiency due to LCK deficiency(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAdistal lck promoter, Jellyfish GFP cDNA, human GH enhancer
    Research applicationFluorescent Proteins/lacZ System
    Immunology and Inflammation Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. As this BIOLOGICAL RESOURCE has been unpublished yet, the recipient is requested to use the BIOLOGICAL RESOURCE as collaborative study with Nakayama Toshinori, and the first publication should include Nakayama Toshinori as a co-author.
    DepositorToshinori Nakayama (Chiba University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Yamashita M, Ukai-Tadenuma M, Miyamoto T, Sugaya K, Hosokawa H, Hasegawa A, Kimura M, Taniguchi M, DeGregori J, Nakayama T.
    Essential role of GATA3 for the maintenance of type 2 helper T (Th2) cytokine production and chromatin remodeling at the Th2 cytokine gene loci.
    J Biol Chem 279(26) 26983-90(2004) 15087456