Strain Data Sheet

RBRC00904

Strain Information

Image
BRC No.RBRC00904
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameC.129S2-Pdcd1<tm1Hon>/HonRbrc (N12)
Former Common namePD-1-KO-N12 (BALB/c)
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strainBALB/cCrSlc
Appearancealbino [A/A b/b c/c]
Strain development文献参照
Strain descriptionPD-1遺伝子はCD28ファミリーに属する免疫抑制受容体であり，活性化したリンパ球に発現し，リンパ球の機能を抑制する．PD-1 (Pdcd1) 遺伝子をノックアウトしたマウスではドナーに用いたマウスの遺伝的背景によってさまざまな自己免疫疾患を発症する．BALB/c背景のPD-1遺伝子ノックアウトマウスは自己免疫性の拡張型心筋症を発症することが示されている．自己免疫疾患制御のメカニズム，その遺伝的要因の関与の解明に有用．
Colony maintenanceN12のライン（BRC No. 00904）はあまり発症がひどくない（ホモ維持可能）。N10のライン（BRC No. 00903）は発症しやすく程度もひどい。
References
Immunological studies on PD-1 deficient mice: implication of PD-1 as a negative regulator for B cell responses.
H Nishimura, N Minato, T Nakano, T Honjo
Int. Immunol., 10, 1563-1572 (1998). 9796923

Development of lupus-like autoimmune diseases by disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor.
H Nishimura, M Nose, H Hiai, N Minato, T Honjo
Immunity, 11, 141-151 (1999). 10485649

Autoimmune dilated cardiomyopathy in PD-1 receptor-deficient mice.
H Nishimura, T Okazaki, Y Tanaka, K Nakatani, M Hara, A Matsumori, S Sasayama, A Mizoguchi, H Hiai, N Minato, T Honjo
Science, 291, 319-322 (2001). 11209085

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pdcd1
MGI:104879		programmed cell death 11Pdcd1<tm1Hon>
MGI:2386184		targeted mutation 1, Tasuku Honjo
  • multiple sclerosis, susceptibility to(MedGEN)

  • systemic lupus erythematosus(DisGeNET)

  • systemic lupus erythematosus, susceptibility to, 2(MedGEN)
    • neo neomycin resistance gene (E. coli)1 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal T cell activation (MP:0001828)

  • abnormal cardiac muscle contractility (MP:0002972)

  • abnormal cardiac output (MP:0001627)

  • abnormal cardiovascular system physiology (MP:0001544)

  • abnormal heart morphology (MP:0000266)
    • more 28 phenotypes
  • abnormal immunoglobulin level (MP:0002490)

  • abnormal leukopoiesis (MP:0005460)

  • abnormal myelopoiesis (MP:0001601)

  • abnormal response to transplant (MP:0005671)

  • arthritis (MP:0002993)

  • cardiac fibrosis (MP:0003141)

  • decreased heart right ventricle wall thickness (MP:0021160)

  • dilated heart ventricle (MP:0008022)

  • enlarged heart (MP:0000274)

  • enlarged liver (MP:0000599)

  • enlarged spleen (MP:0000691)

  • exophthalmos (MP:0002750)

  • glomerulonephritis (MP:0002743)

  • hepatic necrosis (MP:0001654)

  • increased B cell number (MP:0005014)

  • increased B cell proliferation (MP:0005154)

  • increased B-1 B cell number (MP:0004977)

  • increased IgA level (MP:0002495)

  • increased IgG2b level (MP:0008501)

  • increased IgG3 level (MP:0008502)

  • increased IgM level (MP:0002494)

  • increased autoantibody level (MP:0003725)

  • increased inflammatory response (MP:0001846)

  • increased interferon-gamma secretion (MP:0008566)

  • increased lymphocyte cell number (MP:0005013)

  • pancreas necrosis (MP:0009148)

  • premature death (MP:0002083)

  • pulmonary necrosis (MP:0010857)
    • Detailed phenotype data

    Ordering Information

    供与核酸mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse PD-1(immunoglobulin superfamily member) genomic DNA
    Research applicationImmunology and Inflammation Research
    提供条件1. 公表を前提とした学術研究に限る。
    2. 研究成果の公表にあたって寄託者への謝辞の表明並びに寄託者の指定する文献を引用する。Science 291, 319-322 (2001).
    3. 本件リソースの改変体又は本件リソースの生産方法若しくは使用方法に係る特許を出願した場合、その旨を速やかに寄託者に通知する。
    Depositor本庶　佑（京都大学）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Honjo Lab HP
    Genotyping protocol -PCR-
    Mouse of the Month Mar 2005

    BRC mice in Publications

    Ding ZC, Lu X, Yu M, Lemos H, Huang L, Chandler P, Liu K, Walters M, Krasinski A, Mack M, Blazar BR, Mellor AL, Munn DH, Zhou G.
    Immunosuppressive myeloid cells induced by chemotherapy attenuate antitumor CD4+ T-cell responses through the PD-1-PD-L1 axis.
    Cancer Res 74(13) 3441-53(2014) 24780756
    Ding ZC, Huang L, Blazar BR, Yagita H, Mellor AL, Munn DH, Zhou G.
    Polyfunctional CD4⁺ T cells are essential for eradicating advanced B-cell lymphoma after chemotherapy.
    Blood 120(11) 2229-39(2012) 22859605
    Ding ZC, Blazar BR, Mellor AL, Munn DH, Zhou G.
    Chemotherapy rescues tumor-driven aberrant CD4+ T-cell differentiation and restores an activated polyfunctional helper phenotype.
    Blood 115(12) 2397-406(2010) 20118405