RBRC00903

Information1

Image
BRC No.RBRC00903
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameC.129S2-Pdcd1<tm1Hon>/HonRbrc (N10)
Former Common namePD-1-KO-N10 (BALB/c)
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strainBALB/c
Appearancealbino [A/A b/b c/c]
Strain developmentDeveloped by Dr. Tasuku Honjo, Kyoto University
Strain descriptionThe PD1 (programmed cell death 1) gene is an immunoinhibitory receptor that belongs to the CD28 family, and plays a role in the negative control of proliferation, differentiation and class switching of B cells. Homozygous mutant mice exhibit abnormalities in leukopoiesis and immune system which vary considerably depending on the genetic background. The homozygous mutant mice with BALB/c background exhibit autoimmune dilated cardiomyopathy. The mutant mice of the BALB/c-PD1 (N10 line) show severe dilated cardiomyopathy and start to die as early as 5 weeks of age. Meanwhile, the BALB/c-PD1 (N12) line mutant mice exhibit less severe dilated cardiomyopathy and survive longer than N10 line.
Colony maintenanceSibling Mating (Homozygote x Homozygote) Homozygous mutant mice are fertile and viable.
ReferencesScience, 291, 319-322 (2001). 11209085
Immunity, 11, 141-151 (1999). 10485649
Int. Immunol., 10, 1563-1572 (1998). 9796923

Health Report

Examination Date / Room / Rack2020/07/06Room:4-BRack:H
2020/03/30Room:4-BRack:H
2020/01/27Room:4-BRack:H
2019/11/25Room:4-BRack:H
2019/09/30Room:4-BRack:H
2019/07/29Room:4-BRack:H
2019/05/27Room:4-BRack:H
2019/03/25Room:4-BRack:H

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Pdcd1programmed cell death 11Pdcd1<tm1Hon>targeted mutation 1, Tasuku Honjo

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)1mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse PD-1(immunoglobulin superfamily member) genomic DNA
Research applicationImmunology and Inflammation Research
Specific Term and Conditions1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Science 291, 319-322 (2001).
3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
DepositorTasuku Honjo (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Honjo Lab HP
Genotyping protocol -PCR-
Mouse of the Month Mar 2005

BRC mice in Publications

No Data