Strain Data Sheet

RBRC00897

Strain Information

Image
BRC No.RBRC00897
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Aicda<tm1Hon> (N10)/HonRbrc
Former Common nameAID-KO C57BL/6 N10, B6-AID KO#N10, B6.Cg-Aicda<tm1Hon> (N10)/HonRbrc
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
AppearanceBlack [a/a B/B C/C]
Strain development常法にて遺伝子破壊マウスを作る。Genetic backgroundは(C57BL/6 x CBA) x C57BL/6を親として、これに10回C57BL/6へ戻し交配した。Cell, 102, 553-563 (2000).参照
Strain description軽度の液性免疫不全がある。IgG, IgA, IgEを産生しない。
Colony maintenanceSPFであれば、特に飼育、交配は、通常通り可能。
References
Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme.
Muramatsu M, Kinoshita K, Fagarasan S, Yamada S, Shinkai Y, Honjo T
Cell, 102, 553-563 (2000). 11007474

Health Report

Examination Date / Room / Rack2024/04/08Room:3-ARack:I
2024/01/09Room:3-ARack:I
2023/10/10Room:3-ARack:I
2023/07/10Room:3-ARack:I
2023/04/10Room:3-ARack:I
2023/01/10Room:3-ARack:I
2022/10/11Room:3-ARack:I

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Aicdaactivation-induced cytidine deaminase6Aicdatargeted mutation 1, Tasuku Honjo
  • hyper-IgM syndrome type 2(MedGEN)
    • loxPphage P1 loxP6loxP
  • hyper-IgM syndrome type 2(MedGEN)
    • loxPphage P1 loxP6loxP
  • hyper-IgM syndrome type 2(MedGEN)
    • neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)
  • hyper-IgM syndrome type 2(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal class switch recombination(MP:0004816)

  • abnormal somatic hypermutation frequency(MP:0004815)

  • chromosomal instability(MP:0008866)

  • decreased IgA level(MP:0001807)

  • decreased IgG level(MP:0001805)
    • more 7 phenotypes
  • decreased IgG1 level(MP:0008495)

  • decreased IgG2a level(MP:0008496)

  • decreased IgG2b level(MP:0008497)

  • decreased IgG3 level(MP:0008498)

  • increased IgM level(MP:0002494)

  • increased germinal center B cell number(MP:0008177)

  • increased susceptibility to bacterial infection induced morbidity/mortality(MP:0009788)
    • Detailed phenotype data

    Ordering Information

    供与核酸phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Activation-induced cytidine deaminase (AID) genomic DNA
    Research applicationCre/loxP system
    Immunology and Inflammation Research
    提供条件条件を付加する。
    1. 公表を前提とした学術研究に限る。
    2. 研究成果の公表にあたって寄託者への謝辞の表明並びに寄託者の指定する文献を引用する。Cell 102, 553-563 (2000).
    3. 本件リソースの改変体又は本件リソースの生産方法若しくは使用方法に係る特許を出願した場合、その旨を速やかに寄託者に通知する。
    Depositor本庶　佑（京都大学）
    Strain Status生体のアイコン生体
    凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    生体マウスを1~3ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Honjo Lab HP
    Genotyping protocol -PCR-
    Mouse of the Month Dec 2005

    BRC mice in Publications

    Matsumoto M, Sasaki Y, Yasuda K, Takai T, Muramatsu M, Yoshimoto T, Nakanishi K.
    IgG and IgE collaboratively accelerate expulsion of Strongyloides venezuelensis in a primary infection.
    Infect Immun 81(7) 2518-27(2013) 23630966
    Bello A, Müller A, Hirth G, Giebeler LN, Böttcher K, Voigt S, Jungnickel B.
    Cell Cycle-Mediated Regulation of Secondary Ig Diversification.
    J Immunol 210(10) 1508-1518(2023) 37000470
    Kato M, Tsuji-Kawahara S, Kawasaki Y, Kinoshita S, Chikaishi T, Takamura S, Fujisawa M, Kawada A, Miyazawa M.
    Class switch recombination and somatic hypermutation of virus-neutralizing antibodies are not essential for control of friend retrovirus infection.
    J Virol 89(2) 1468-73(2015) 25378499
    Luo Y, Zhang X, Chen R, Li R, Liu Y, Zhang J, Liu Q, Si M, Liu J, Wu B, Wang X, Wu S, Zhang Y, Zhang X, Guo D, He X, Pan T, Zhang H.
    USP10 regulates B cell response to SARS-CoV-2 or HIV-1 nanoparticle vaccines through deubiquitinating AID.
    Signal Transduct Target Ther 7(1) 7(2022) 34983926