Strain Data Sheet

RBRC00897

Strain Information

Image
BRC No.RBRC00897
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Aicda<tm1Hon> (N10)/HonRbrc
Former Common nameAID-KO C57BL/6 N10, B6-AID KO#N10, B6.Cg-Aicda<tm1Hon> (N10)/HonRbrc
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
AppearanceBlack [a/a B/B C/C]
Strain developmentDeveloped by Tasuku Honjo, Graduate School of Medicine, Kyoto University. The construct was electoroporated into TT2 ES cells derived from (C57BL/6 x CBA)F1. The mutant mice were backcrossed to C57BL/6N.
Strain descriptionAID (Aicda, activation-induced cytidine deaminase) knockout mice. Exon 2 and part of exon 3 were replaced with a neomycin resistance cassette. Aid, Activation-Induced cytidine Deaminase is a molecule which specifically expressed in B lymphocytes after antigen stimulation. AID deficiency caused a complete defect in class switching and showed a hyper-IgM phenotype with enlarged germinal centers containing strongly activated B cells before or after immunization. Accumulation of IgM positive B cell is remarkable in the gut lamina propria. Furthermore, it is suggested that a mutation of the Aid gene was a cause of the hereditary immune deficiency disorder, type-2 hyper-IgM syndrome (HIGM2) in human. As well as class switch recombination, it was also indicated that somatic hypermutation of an immunoglobulin variable region gene and gene conversion do not occur at all in the Aid deficiency mice. Aid gene is a molecule serving as a central role in antibody gene variety acquisition mechanism of B cell.
Colony maintenanceHomozygote x Homozygote. Homozygous mutant mice are viable and fertile.
References
Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme.
Muramatsu M, Kinoshita K, Fagarasan S, Yamada S, Shinkai Y, Honjo T
Cell, 102, 553-563 (2000). 11007474

Health Report

Examination Date / Room / Rack2024/04/08Room:3-ARack:I
2024/01/09Room:3-ARack:I
2023/10/10Room:3-ARack:I
2023/07/10Room:3-ARack:I
2023/04/10Room:3-ARack:I
2023/01/10Room:3-ARack:I
2022/10/11Room:3-ARack:I

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Aicdaactivation-induced cytidine deaminase6Aicdatargeted mutation 1, Tasuku Honjo
  • hyper-IgM syndrome type 2(MedGEN)
    • loxPphage P1 loxP6loxP
  • hyper-IgM syndrome type 2(MedGEN)
    • loxPphage P1 loxP6loxP
  • hyper-IgM syndrome type 2(MedGEN)
    • neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)
  • hyper-IgM syndrome type 2(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal class switch recombination(MP:0004816)

  • abnormal somatic hypermutation frequency(MP:0004815)

  • chromosomal instability(MP:0008866)

  • decreased IgA level(MP:0001807)

  • decreased IgG level(MP:0001805)
    • more 7 phenotypes
  • decreased IgG1 level(MP:0008495)

  • decreased IgG2a level(MP:0008496)

  • decreased IgG2b level(MP:0008497)

  • decreased IgG3 level(MP:0008498)

  • increased IgM level(MP:0002494)

  • increased germinal center B cell number(MP:0008177)

  • increased susceptibility to bacterial infection induced morbidity/mortality(MP:0009788)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Activation-induced cytidine deaminase (AID) genomic DNA
    Research applicationCre/loxP system
    Immunology and Inflammation Research
    Specific Term and Conditions1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
    2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Cell 102, 553-563 (2000).
    3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
    DepositorTasuku Honjo (Kyoto University)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Honjo Lab HP
    Genotyping protocol -PCR-
    Mouse of the Month Dec 2005

    BRC mice in Publications

    Matsumoto M, Sasaki Y, Yasuda K, Takai T, Muramatsu M, Yoshimoto T, Nakanishi K.
    IgG and IgE collaboratively accelerate expulsion of Strongyloides venezuelensis in a primary infection.
    Infect Immun 81(7) 2518-27(2013) 23630966
    Bello A, Müller A, Hirth G, Giebeler LN, Böttcher K, Voigt S, Jungnickel B.
    Cell Cycle-Mediated Regulation of Secondary Ig Diversification.
    J Immunol 210(10) 1508-1518(2023) 37000470
    Kato M, Tsuji-Kawahara S, Kawasaki Y, Kinoshita S, Chikaishi T, Takamura S, Fujisawa M, Kawada A, Miyazawa M.
    Class switch recombination and somatic hypermutation of virus-neutralizing antibodies are not essential for control of friend retrovirus infection.
    J Virol 89(2) 1468-73(2015) 25378499
    Luo Y, Zhang X, Chen R, Li R, Liu Y, Zhang J, Liu Q, Si M, Liu J, Wu B, Wang X, Wu S, Zhang Y, Zhang X, Guo D, He X, Pan T, Zhang H.
    USP10 regulates B cell response to SARS-CoV-2 or HIV-1 nanoparticle vaccines through deubiquitinating AID.
    Signal Transduct Target Ther 7(1) 7(2022) 34983926