Strain Data Sheet

RBRC00892

Strain Information

Image
BRC No.RBRC00892
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(CAG-EGFP,-Aicda)20Hon/HonRbrc
Former Common nameAID-conditional Transgenic mouse line 20
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Tasuku Honjo and Ilmi Okazaki at Kyoto University in 2002.
Strain descriptionThese transgenic mice constitutively express GFP under control of CAG promoter. When crossed with a Cre recombinase-expressing strain, GFP expression is replaced with mouse AID expression in tissue expressing Cre. AID, Activation-Induced cytidine Deaminase is a molecule which specifically expressed in B lymphocytes after antigen stimulation, playing a central role in acquisition for B cell of antibody gene variety.
Colony maintenanceBackcross to C57BL/6 (Hemizygote x C57BL/6CrSlc)
References
Efficient selection for high-expression transfectants with a novel eukaryotic vector.
H Niwa, K Yamamura, J Miyazaki
Gene, 108(2):193-9 (1991). 1660837

Negative regulation of activation-induced cytidine deaminase in B cells.
Taro Muto, Il-mi Okazaki, Shuichi Yamada, Yoshimasa Tanaka, Kazuo Kinoshita, Masamichi Muramatsu, Hitoshi Nagaoka, Tasuku Honjo
Proc. Natl. Acad. Sci. USA, 103, 2752-2757 (2006). 16477013

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Aicda
MGI:1342279		activation-induced cytidine deaminaseUNAicda
  • hyper-IgM syndrome type 2(MedGEN)
    • GFP Green Fluorescent Protein (Aequorea victoria)UN CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
    loxP phage P1 loxPUNloxP
    loxP phage P1 loxPUNloxP

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • normal phenotype (MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP sites, Jellyfish GFP cDNA, mouse Aicda cDNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Immunology and Inflammation Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 103, 2752-2757 (2006). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
    DepositorTasuku Honjo (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Honjo Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data