Strain Data Sheet

RBRC00886

Strain Information

Image
BRC No.RBRC00886
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;B6C3-Tg(Acro3-EGFP)01Osb
Former Common nameB6;C3 Tg(acro3-EGFP)01Osb (Nov. 2011), B6C3F1; B6C3F1 TgN(acro3-EGFP)/Osb01
H-2 Haplotype
ES Cell line
Background strainB6C3F1[C57BL/6N and C3H/HeN]
Appearanceblack [a/a B/B C/C]
Strain development開発者:岡部勝先生機関名:大阪大学遺伝情報実験センターBCF1 その後不明。
Strain description精子の先体部分が480 nmで励起すれば光る。
Colony maintenance特になし。
References
Real-time observation of acrosomal dispersal from mouse sperm using GFP as a marker protein.
Nakanishi T, Ikawa M, Yamada S, Parvinen M, Baba T, Nishimune Y, Okabe M
FEBS Lett., 449, 277-283 (1999). 10338148

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFPGreen Fluorescent Protein (Aequorea victoria)UNmouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
  • Detailed phenotype data

    Ordering Information

    供与核酸mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide, Jellyfish GFP cDNA, rabbit beta-globin polyadenylation signal
    Research applicationFluorescent Proteins/lacZ System
    提供条件利用者は事前に寄託者の提供承諾書を得る。研究成果の公表にあたって寄託者の指定する文献を引用する。FEBS Lett., 449, 277-283 (1999). 非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること(https://www.ccb.osaka-u.ac.jp/en/)。5年経過後も使用を希望するときは改めて寄託者から承諾を得るものとする。
    Depositor岡部 勝(国立大学法人大阪大学)
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP, Co-creation Bureau, Osaka University HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Yamanaka H, Komeya M, Nakamura H, Sanjo H, Sato T, Yao M, Kimura H, Fujii T, Ogawa T.
    A monolayer microfluidic device supporting mouse spermatogenesis with improved visibility.
    Biochem Biophys Res Commun 500(4) 885-891(2018) 29705697
    Gohbara A, Katagiri K, Sato T, Kubota Y, Kagechika H, Araki Y, Araki Y, Ogawa T.
    In vitro murine spermatogenesis in an organ culture system.
    Biol Reprod 83(2) 261-7(2010) 20393168
    Hirano K, Nonami Y, Nakamura Y, Sato T, Sato T, Ishiguro KI, Ogawa T, Yoshida S.
    Temperature sensitivity of DNA double-strand break repair underpins heat-induced meiotic failure in mouse spermatogenesis.
    Commun Biol 5(1) 504(2022) 35618762
    Okugi K, Kuwahara N, Yanome N, Yamada K, Ito T, Takano A, Ohira S, Nagai A, Toné S.
    An in vitro system for experimentally induced cryptorchidism.
    Histochem Cell Biol 157(3) 297-307(2022) 35190876
    Fukunaga H, Kaminaga K, Sato T, Watanabe R, Ogawa T, Yokoya A, Prise KM.
    The Tissue-Sparing Effect of Spatially Fractionated X-rays for Maintaining Spermatogenesis: A Radiobiological Approach for the Preservation of Male Fertility after Radiotherapy.
    J Clin Med 9(4) (2020) 32290436
    Sato T, Katagiri K, Yokonishi T, Kubota Y, Inoue K, Ogonuki N, Matoba S, Ogura A, Ogawa T.
    In vitro production of fertile sperm from murine spermatogonial stem cell lines.
    Nat Commun 2 472(2011) 21915114
    Sato T, Katagiri K, Gohbara A, Inoue K, Ogonuki N, Ogura A, Kubota Y, Ogawa T.
    In vitro production of functional sperm in cultured neonatal mouse testes.
    Nature 471(7339) 504-7(2011) 21430778
    Fukunaga H, Kaminaga K, Sato T, Usami N, Watanabe R, Butterworth KT, Ogawa T, Yokoya A, Prise KM.
    Application of an Ex Vivo Tissue Model to Investigate Radiobiological Effects on Spermatogenesis.
    Radiat Res 189(6) 661-667(2018) 29595376
    Nakagiri H, Ogawa T, Ikeda N, Terasaka S, Nukada Y, Miyazawa M.
    Application of testicular organ culture system for the evaluation of spermatogenesis impairment.
    Sci Rep 14(1) 21581(2024) 39285184
    Hashimoto K, Odaka H, Ishikawa-Yamauchi Y, Nagata S, Nakamura H, Kimura H, Sato T, Makiyama K, Ogawa T.
    Culture-space control is effective in promoting haploid cell formation and spermiogenesis in vitro in neonatal mice.
    Sci Rep 13(1) 12354(2023) 37524742
    Komeya M, Hayashi K, Nakamura H, Yamanaka H, Sanjo H, Kojima K, Sato T, Yao M, Kimura H, Fujii T, Ogawa T.
    Pumpless microfluidic system driven by hydrostatic pressure induces and maintains mouse spermatogenesis in vitro.
    Sci Rep 7(1) 15459(2017) 29133858
    Komeya M, Kimura H, Nakamura H, Yokonishi T, Sato T, Kojima K, Hayashi K, Katagiri K, Yamanaka H, Sanjo H, Yao M, Kamimura S, Inoue K, Ogonuki N, Ogura A, Fujii T, Ogawa T.
    Long-term ex vivo maintenance of testis tissues producing fertile sperm in a microfluidic device.
    Sci Rep 6 21472(2016) 26892171