Strain Information | |
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Image | |
BRC No. | RBRC00886 |
Type | Transgene |
Species | Mus musculus |
Strain name | B6;B6C3-Tg(Acro3-EGFP)01Osb |
Former Common name | B6;C3 Tg(acro3-EGFP)01Osb (Nov. 2011), B6C3F1; B6C3F1 TgN(acro3-EGFP)/Osb01 |
H-2 Haplotype | |
ES Cell line | |
Background strain | B6C3F1[C57BL/6N and C3H/HeN] |
Appearance | black [a/a B/B C/C] |
Strain development | 開発者:岡部勝先生機関名:大阪大学遺伝情報実験センターBCF1 その後不明。 |
Strain description | 精子の先体部分が480 nmで励起すれば光る。 |
Colony maintenance | 特になし。 |
References | FEBS Lett., 449, 277-283 (1999). 10338148 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GFPGreen Fluorescent Protein (Aequorea victoria)UNmouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide |
Ordering Information | |
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供与核酸 | mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide, Jellyfish GFP cDNA, rabbit beta-globin polyadenylation signal |
Research application | Fluorescent Proteins/lacZ System |
提供条件 | 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。 研究成果の公表にあたって寄託者の指定する文献を引用する。FEBS Lett., 449, 277-283 (1999). 非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること(https://www.ccb.osaka-u.ac.jp/en/)。5年経過後も使用を希望するときは改めて寄託者から承諾を得るものとする。 |
Depositor | 岡部 勝(国立大学法人大阪大学) |
Strain Status | 凍結胚 凍結精子 |
Strain Availability | 凍結胚より作出したマウスを2~4ヶ月以内に提供可能 凍結精子を1ヶ月以内に提供可能 凍結胚を1ヶ月以内に提供可能 |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Yamanaka H, Komeya M, Nakamura H, Sanjo H, Sato T, Yao M, Kimura H, Fujii T, Ogawa T. A monolayer microfluidic device supporting mouse spermatogenesis with improved visibility. Biochem Biophys Res Commun 500(4) 885-891(2018) 29705697 Gohbara A, Katagiri K, Sato T, Kubota Y, Kagechika H, Araki Y, Araki Y, Ogawa T. In vitro murine spermatogenesis in an organ culture system. Biol Reprod 83(2) 261-7(2010) 20393168 Hirano K, Nonami Y, Nakamura Y, Sato T, Sato T, Ishiguro KI, Ogawa T, Yoshida S. Temperature sensitivity of DNA double-strand break repair underpins heat-induced meiotic failure in mouse spermatogenesis. Commun Biol 5(1) 504(2022) 35618762 Okugi K, Kuwahara N, Yanome N, Yamada K, Ito T, Takano A, Ohira S, Nagai A, Toné S. An in vitro system for experimentally induced cryptorchidism. Histochem Cell Biol 157(3) 297-307(2022) 35190876 Fukunaga H, Kaminaga K, Sato T, Watanabe R, Ogawa T, Yokoya A, Prise KM. The Tissue-Sparing Effect of Spatially Fractionated X-rays for Maintaining Spermatogenesis: A Radiobiological Approach for the Preservation of Male Fertility after Radiotherapy. J Clin Med 9(4) (2020) 32290436 Sato T, Katagiri K, Yokonishi T, Kubota Y, Inoue K, Ogonuki N, Matoba S, Ogura A, Ogawa T. In vitro production of fertile sperm from murine spermatogonial stem cell lines. Nat Commun 2 472(2011) 21915114 Sato T, Katagiri K, Gohbara A, Inoue K, Ogonuki N, Ogura A, Kubota Y, Ogawa T. In vitro production of functional sperm in cultured neonatal mouse testes. Nature 471(7339) 504-7(2011) 21430778 Fukunaga H, Kaminaga K, Sato T, Usami N, Watanabe R, Butterworth KT, Ogawa T, Yokoya A, Prise KM. Application of an Ex Vivo Tissue Model to Investigate Radiobiological Effects on Spermatogenesis. Radiat Res 189(6) 661-667(2018) 29595376 Hashimoto K, Odaka H, Ishikawa-Yamauchi Y, Nagata S, Nakamura H, Kimura H, Sato T, Makiyama K, Ogawa T. Culture-space control is effective in promoting haploid cell formation and spermiogenesis in vitro in neonatal mice. Sci Rep 13(1) 12354(2023) 37524742 Komeya M, Hayashi K, Nakamura H, Yamanaka H, Sanjo H, Kojima K, Sato T, Yao M, Kimura H, Fujii T, Ogawa T. Pumpless microfluidic system driven by hydrostatic pressure induces and maintains mouse spermatogenesis in vitro. Sci Rep 7(1) 15459(2017) 29133858 Komeya M, Kimura H, Nakamura H, Yokonishi T, Sato T, Kojima K, Hayashi K, Katagiri K, Yamanaka H, Sanjo H, Yao M, Kamimura S, Inoue K, Ogonuki N, Ogura A, Fujii T, Ogawa T. Long-term ex vivo maintenance of testis tissues producing fertile sperm in a microfluidic device. Sci Rep 6 21472(2016) 26892171 |