Strain Data Sheet

RBRC00886

update : 2024-04-19

Strain Information
Image
BRC No.	RBRC00886
Type	Transgene
Species	Mus musculus
Strain name	B6;B6C3-Tg(Acro3-EGFP)01Osb
Former Common name	B6;C3 Tg(acro3-EGFP)01Osb (Nov. 2011), B6C3F1; B6C3F1 TgN(acro3-EGFP)/Osb01
H-2 Haplotype
ES Cell line
Background strain	B6C3F1[C57BL/6N and C3H/HeN]
Appearance	black [a/a B/B C/C]
Strain development	開発者：岡部勝先生機関名：大阪大学遺伝情報実験センターBCF1　その後不明。
Strain description	精子の先体部分が480 nmで励起すれば光る。
Colony maintenance	特になし。
References	FEBS Lett., 449, 277-283 (1999). 10338148

Health Report
Examination Date / Room / Rack

Gene
Gene info	Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GFPGreen Fluorescent Protein (Aequorea victoria)UNmouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide

Ordering Information
供与核酸	mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide, Jellyfish GFP cDNA, rabbit beta-globin polyadenylation signal
Research application	Fluorescent Proteins/lacZ System
提供条件	条件を付加する。利用者は事前に寄託者の提供承諾書を得る。研究成果の公表にあたって寄託者の指定する文献を引用する。FEBS Lett., 449, 277-283 (1999). 非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること（https://www.ccb.osaka-u.ac.jp/en/）。5年経過後も使用を希望するときは改めて寄託者から承諾を得るものとする。
Depositor	岡部　勝（国立大学法人大阪大学）
Strain Status	凍結胚凍結精子
Strain Availability	凍結胚より作出したマウスを2~4ヶ月以内に提供可能凍結精子を1ヶ月以内に提供可能凍結胚を1ヶ月以内に提供可能
Additional Info.	Necessary documents for ordering: Approval form (Japanese / English) Order form (Japanese / English) Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English) Acceptance of responsibility for living modified organism (Japanese / English) Lab HP Genotyping protocol -PCR-

BRC mice in Publications

Yamanaka H, Komeya M, Nakamura H, Sanjo H, Sato T, Yao M, Kimura H, Fujii T, Ogawa T. A monolayer microfluidic device supporting mouse spermatogenesis with improved visibility. Biochem Biophys Res Commun 500(4) 885-891(2018) 29705697 Gohbara A, Katagiri K, Sato T, Kubota Y, Kagechika H, Araki Y, Araki Y, Ogawa T. In vitro murine spermatogenesis in an organ culture system. Biol Reprod 83(2) 261-7(2010) 20393168 Hirano K, Nonami Y, Nakamura Y, Sato T, Sato T, Ishiguro KI, Ogawa T, Yoshida S. Temperature sensitivity of DNA double-strand break repair underpins heat-induced meiotic failure in mouse spermatogenesis. Commun Biol 5(1) 504(2022) 35618762 Okugi K, Kuwahara N, Yanome N, Yamada K, Ito T, Takano A, Ohira S, Nagai A, Toné S. An in vitro system for experimentally induced cryptorchidism. Histochem Cell Biol 157(3) 297-307(2022) 35190876 Fukunaga H, Kaminaga K, Sato T, Watanabe R, Ogawa T, Yokoya A, Prise KM. The Tissue-Sparing Effect of Spatially Fractionated X-rays for Maintaining Spermatogenesis: A Radiobiological Approach for the Preservation of Male Fertility after Radiotherapy. J Clin Med 9(4) (2020) 32290436 Sato T, Katagiri K, Yokonishi T, Kubota Y, Inoue K, Ogonuki N, Matoba S, Ogura A, Ogawa T. In vitro production of fertile sperm from murine spermatogonial stem cell lines. Nat Commun 2 472(2011) 21915114 Sato T, Katagiri K, Gohbara A, Inoue K, Ogonuki N, Ogura A, Kubota Y, Ogawa T. In vitro production of functional sperm in cultured neonatal mouse testes. Nature 471(7339) 504-7(2011) 21430778 Fukunaga H, Kaminaga K, Sato T, Usami N, Watanabe R, Butterworth KT, Ogawa T, Yokoya A, Prise KM. Application of an Ex Vivo Tissue Model to Investigate Radiobiological Effects on Spermatogenesis. Radiat Res 189(6) 661-667(2018) 29595376 Hashimoto K, Odaka H, Ishikawa-Yamauchi Y, Nagata S, Nakamura H, Kimura H, Sato T, Makiyama K, Ogawa T. Culture-space control is effective in promoting haploid cell formation and spermiogenesis in vitro in neonatal mice. Sci Rep 13(1) 12354(2023) 37524742 Komeya M, Hayashi K, Nakamura H, Yamanaka H, Sanjo H, Kojima K, Sato T, Yao M, Kimura H, Fujii T, Ogawa T. Pumpless microfluidic system driven by hydrostatic pressure induces and maintains mouse spermatogenesis in vitro. Sci Rep 7(1) 15459(2017) 29133858 Komeya M, Kimura H, Nakamura H, Yokonishi T, Sato T, Kojima K, Hayashi K, Katagiri K, Yamanaka H, Sanjo H, Yao M, Kamimura S, Inoue K, Ogonuki N, Ogura A, Fujii T, Ogawa T. Long-term ex vivo maintenance of testis tissues producing fertile sperm in a microfluidic device. Sci Rep 6 21472(2016) 26892171

BRC mice in Publications

Yamanaka H, Komeya M, Nakamura H, Sanjo H, Sato T, Yao M, Kimura H, Fujii T, Ogawa T.
A monolayer microfluidic device supporting mouse spermatogenesis with improved visibility.
Biochem Biophys Res Commun 500(4) 885-891(2018) 29705697

Gohbara A, Katagiri K, Sato T, Kubota Y, Kagechika H, Araki Y, Araki Y, Ogawa T.
In vitro murine spermatogenesis in an organ culture system.
Biol Reprod 83(2) 261-7(2010) 20393168

Hirano K, Nonami Y, Nakamura Y, Sato T, Sato T, Ishiguro KI, Ogawa T, Yoshida S.
Temperature sensitivity of DNA double-strand break repair underpins heat-induced meiotic failure in mouse spermatogenesis.
Commun Biol 5(1) 504(2022) 35618762

Okugi K, Kuwahara N, Yanome N, Yamada K, Ito T, Takano A, Ohira S, Nagai A, Toné S.
An in vitro system for experimentally induced cryptorchidism.
Histochem Cell Biol 157(3) 297-307(2022) 35190876

Fukunaga H, Kaminaga K, Sato T, Watanabe R, Ogawa T, Yokoya A, Prise KM.
The Tissue-Sparing Effect of Spatially Fractionated X-rays for Maintaining Spermatogenesis: A Radiobiological Approach for the Preservation of Male Fertility after Radiotherapy.
J Clin Med 9(4) (2020) 32290436

Sato T, Katagiri K, Yokonishi T, Kubota Y, Inoue K, Ogonuki N, Matoba S, Ogura A, Ogawa T.
In vitro production of fertile sperm from murine spermatogonial stem cell lines.
Nat Commun 2 472(2011) 21915114

Sato T, Katagiri K, Gohbara A, Inoue K, Ogonuki N, Ogura A, Kubota Y, Ogawa T.
In vitro production of functional sperm in cultured neonatal mouse testes.
Nature 471(7339) 504-7(2011) 21430778

Fukunaga H, Kaminaga K, Sato T, Usami N, Watanabe R, Butterworth KT, Ogawa T, Yokoya A, Prise KM.
Application of an Ex Vivo Tissue Model to Investigate Radiobiological Effects on Spermatogenesis.
Radiat Res 189(6) 661-667(2018) 29595376

Hashimoto K, Odaka H, Ishikawa-Yamauchi Y, Nagata S, Nakamura H, Kimura H, Sato T, Makiyama K, Ogawa T.
Culture-space control is effective in promoting haploid cell formation and spermiogenesis in vitro in neonatal mice.
Sci Rep 13(1) 12354(2023) 37524742

Komeya M, Hayashi K, Nakamura H, Yamanaka H, Sanjo H, Kojima K, Sato T, Yao M, Kimura H, Fujii T, Ogawa T.
Pumpless microfluidic system driven by hydrostatic pressure induces and maintains mouse spermatogenesis in vitro.
Sci Rep 7(1) 15459(2017) 29133858

Komeya M, Kimura H, Nakamura H, Yokonishi T, Sato T, Kojima K, Hayashi K, Katagiri K, Yamanaka H, Sanjo H, Yao M, Kamimura S, Inoue K, Ogonuki N, Ogura A, Fujii T, Ogawa T.
Long-term ex vivo maintenance of testis tissues producing fertile sperm in a microfluidic device.
Sci Rep 6 21472(2016) 26892171

Strain Information

Health Report

Gene

Ordering Information

BRC mice in Publications