Strain Information | |
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Image | |
BRC No. | RBRC00868 |
Type | Transgene |
Species | Mus musculus |
Strain name | B6.Cg-Tg(GOF18/EGFP)11Ymat |
Former Common name | GOF18-GFP line11 transgenic mouse, B6.Cg-Tg(GOF18/EGFP)11Ymat |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6JJcl |
Appearance | black |
Strain development | Developed by Hans R. Shoeler University of Pennsylvania and Yasuhisa Mastui, Research Institute Oasaka Medical Center of Maternal and Child Health in 1997. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mutant mice were backcrossed to C57BL/6 over 3 times (RBRC00868 and RBRC01198). Backcrossed 129 for 7 time (RBRC01198) (by Atsuo Ogura, RIKEN BioResrouce center). |
Strain description | Oct-4(Pou5f1)/EGFP transgenic mice carrying EGFP cDNA under the control of the 18kb Oct-4 genomic fragment (Genomic Oct-4 Fragment of 18) (Dev Growth Differ 1999). Oct-4 gene expresses in undifferenciated spermatogonia at prepubertal stages. This strain is useful to detect primordial germ cells with green fluorescence protein by the PGC-specific promoter. Tg line including the proximal enhancer (PE) of GOF18 (RBRC00868), Tg line lacking the proximal enhancer (PE) (RBRC00869 and RBRC01198). |
Colony maintenance | Carrier x Noncarrier [C57BL/6JJcl] |
References | Germline-specific expression of the Oct-4/green fluorescent protein (GFP) transgene in mice. Yoshimizu T, Sugiyama N, De Felice M, Yeom Y I, Ohbo K, Masuko K, Obinata M, Abe K, Scholer H R, Matsui Y Dev. Growth Differ., 41, 675-684 (1999). 10646797 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
EGFP | Enhanced Green Fluorescent Protein (Aequorea victoria) | UN | EGFP | mouse Pou5f1 promoter |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | mouse Pou5f1 (Oct3/4) promoter, Jellyfish EGFP cDNA |
Research application | Developmental Biology Research Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Growth. Differ., 41, 675-684 (1999). |
Depositor | Yasuhisa Matsui (Research Institute, Osaka Medical Center for Maternal and Child Health) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |