Strain Data Sheet

RBRC00858

update : 2024-04-19

Strain Information
Image
BRC No.	RBRC00858
Type	Targeted Mutation
Species	Mus musculus
Strain name	B6;129S6-Bcl2l12/Irf3<tm1Ttg>/TtgRbrc
Former Common name	IRF-3 KO/B6; IRF-3 Knockout mice, C57BL/6J
H-2 Haplotype
ES Cell line	CCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain	C57BL/6JJcl
Appearance	black
Strain development	東京大学大学院医学系研究科・谷口維紹先生。CCE/EK.CCE ES細胞を用いて作出。C57BL/6セミコンジェニック背景。
Strain description	IRF-3遺伝子のノックアウトマウス。インターフェロン (IFN) シグナル伝達系は免疫系の制御に極めて重要な働きを果たしており、IRFファミリーはIFN遺伝子の転写制御因子として知られている。IRFファミリーには少なくとも9つ存在していることが報告されており、IRFファミリー転写因子欠損マウスを用いた解析はこれらIRFファミリー転写因子の分子制御を解明する上で有用である。IRF-3はIRF-7と共にウィルス感染によって直接活性化されるINF-α/β遺伝子の発現誘導に関わる必須因子であり、ウィルス感染によって直接活性化されることが知られている。IRF-3欠損マウスではI型インターフェロン (INF-αとINF-β) の生産が著しく低下し、抗ウィルス免疫反応が低下する。
Colony maintenance	Homozygote x Homozygote
References	Immunity, 13, 539-548 (2000). 11070172

Health Report
Examination Date / Room / Rack	2023/08/28Room:4-CRack:A 2023/05/29Room:4-CRack:A 2023/02/27Room:4-CRack:A 2022/11/29Room:4-CRack:A 2022/08/29Room:4-CRack:A 2022/05/31Room:4-CRack:A

Gene
Gene info	Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Irf3interferon regulatory factor 37Irf3<tm1Ttg>targeted mutation 1, Tadatsugu TaniguchiC920001K05Rik, IRF-3 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Bcl2l12BCL2-like 12 (proline rich)7Bcl2l12<tm1Ttg>targeted mutation 1, Tadatsugu TaniguchiBcl2-L12, Bcl-L12 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)7neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Gene

Gene info

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter

Irf3interferon regulatory factor 37Irf3<tm1Ttg>targeted mutation 1, Tadatsugu TaniguchiC920001K05Rik, IRF-3

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter

Bcl2l12BCL2-like 12 (proline rich)7Bcl2l12<tm1Ttg>targeted mutation 1, Tadatsugu TaniguchiBcl2-L12, Bcl-L12

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter

neoneomycin resistance gene (E. coli)7neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information
供与核酸	mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse IRF-3 genomic DNA, mouse Bcl2l12 genomic DNA
Research application	Immunology and Inflammation Research
提供条件	条件を付加する。利用者は事前に寄託者の提供承諾書を得る。研究成果の公表にあたって謝辞の表明を必要とする。利用者は使用目的を寄託者に通知し、この目的の範囲内でのみ使用し、結果を通知する。
Depositor	谷口　維紹（国立大学法人東京大学）
Strain Status	凍結胚凍結精子
Strain Availability	凍結胚より作出したマウスを2~4ヶ月以内に提供可能凍結精子を1ヶ月以内に提供可能凍結胚を1ヶ月以内に提供可能
Additional Info.	Necessary documents for ordering: Approval form (Japanese / English) Order form (Japanese / English) Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English) Acceptance of responsibility for living modified organism (Japanese / English) Genotyping protocol -PCR- Mouse of the Month May 2005 Genetic Background

BRC mice in Publications

Foss MH, Stevens SL, Jin H, Allen EM, Nelson D, DeFilippis V, Nilsen A, Stenzel-Poore MP. Identification and Characterization of Small-Molecule IRF3-Dependent Immune Activators for Pharmaceutical Development. ACS Chem Biol 17(5) 1073-1081(2022) 35471821 Smith H, Liu XY, Dai L, Goh ET, Chan AT, Xi J, Seh CC, Qureshi IA, Lescar J, Ruedl C, Gourlay R, Morton S, Hough J, McIver EG, Cohen P, Cheung PC. The role of TBK1 and IKKε in the expression and activation of Pellino 1. Biochem J 434(3) 537-48(2011) 21204785 Goriely S, Molle C, Nguyen M, Albarani V, Haddou NO, Lin R, De Wit D, Flamand V, Willems F, Goldman M. Interferon regulatory factor 3 is involved in Toll-like receptor 4 (TLR4)- and TLR3-induced IL-12p35 gene activation. Blood 107(3) 1078-84(2006) 16219795 Zhang SM, Zhu LH, Li ZZ, Wang PX, Chen HZ, Guan HJ, Jiang DS, Chen K, Zhang XF, Tian S, Yang D, Zhang XD, Li H. Interferon regulatory factor 3 protects against adverse neo-intima formation. Cardiovasc Res 102(3) 469-79(2014) 24596398 Benmerzoug S, Bounab B, Rose S, Gosset D, Biet F, Cochard T, Xavier A, Rouxel N, Fauconnier L, Horsnell WGC, Ryffel B, Togbe D, Quesniaux VFJ. Sterile Lung Inflammation Induced by Silica Exacerbates Mycobacterium tuberculosis Infection via STING-Dependent Type 2 Immunity. Cell Rep 27(9) 2649-2664.e5(2019) 31141689 Dogusan Z, García M, Flamez D, Alexopoulou L, Goldman M, Gysemans C, Mathieu C, Libert C, Eizirik DL, Rasschaert J. Double-stranded RNA induces pancreatic beta-cell apoptosis by activation of the toll-like receptor 3 and interferon regulatory factor 3 pathways. Diabetes 57(5) 1236-45(2008) 18223009 Sin WX, Yeong JP, Lim TJF, Su IH, Connolly JE, Chin KC. IRF-7 Mediates Type I IFN Responses in Endotoxin-Challenged Mice. Front Immunol 11 640(2020) 32373120 Loi P, Yuan Q, Torres D, Delbauve S, Laute MA, Lalmand MC, Pétein M, Goriely S, Goldman M, Flamand V. Interferon regulatory factor 3 deficiency leads to interleukin-17-mediated liver ischemia-reperfusion injury. Hepatology 57(1) 351-61(2013) 22911673 Prantner D, Sikes JD, Hennings L, Savenka AV, Basnakian AG, Nagarajan UM. Interferon regulatory transcription factor 3 protects mice from uterine horn pathology during Chlamydia muridarum genital infection. Infect Immun 79(10) 3922-33(2011) 21788382 Marichal T, Bedoret D, Mesnil C, Pichavant M, Goriely S, Trottein F, Cataldo D, Goldman M, Lekeux P, Bureau F, Desmet CJ. Interferon response factor 3 is essential for house dust mite-induced airway allergy. J Allergy Clin Immunol 126(4) 836-844.e13(2010) 20673978 Ishii T, Hosoki K, Nikura Y, Yamashita N, Nagase T, Yamashita N. IFN Regulatory Factor 3 Potentiates Emphysematous Aggravation by Lipopolysaccharide. J Immunol 198(9) 3637-3649(2017) 28363903 Prantner D, Darville T, Nagarajan UM. Stimulator of IFN gene is critical for induction of IFN-beta during Chlamydia muridarum infection. J Immunol 184(5) 2551-60(2010) 20107183 Molle C, Goldman M, Goriely S. Critical role of the IFN-stimulated gene factor 3 complex in TLR-mediated IL-27p28 gene expression revealing a two-step activation process. J Immunol 184(4) 1784-92(2010) 20083668 Molle C, Nguyen M, Flamand V, Renneson J, Trottein F, De Wit D, Willems F, Goldman M, Goriely S. IL-27 synthesis induced by TLR ligation critically depends on IFN regulatory factor 3. J Immunol 178(12) 7607-15(2007) 17548596 Liu Y, Mo CF, Luo XY, Li H, Guo HJ, Sun H, Hu S, Li LM, Wang YT, Yang SX, Chang S, Zou Q. Activation of Toll-Like Receptor 3 Induces Interleukin-1 Receptor Antagonist Expression by Activating the Interferon Regulatory Factor 3. J Innate Immun 12(4) 304-320(2020) 31865314 Stevens SL, Leung PY, Vartanian KB, Gopalan B, Yang T, Simon RP, Stenzel-Poore MP. Multiple preconditioning paradigms converge on interferon regulatory factor-dependent signaling to promote tolerance to ischemic brain injury. J Neurosci 31(23) 8456-63(2011) 21653850 Marsh B, Stevens SL, Packard AE, Gopalan B, Hunter B, Leung PY, Harrington CA, Stenzel-Poore MP. Systemic lipopolysaccharide protects the brain from ischemic injury by reprogramming the response of the brain to stroke: a critical role for IRF3. J Neurosci 29(31) 9839-49(2009) 19657036 Christensen JE, Fenger C, Issazadeh-Navikas S, Krug A, Liljestrøm P, Goriely S, Paludan SR, Finsen B, Christensen JP, Thomsen AR. Differential impact of interferon regulatory factor 7 in initiation of the type I interferon response in the lymphocytic choriomeningitis virus-infected central nervous system versus the periphery. J Virol 86(13) 7384-92(2012) 22514347 Zhou S, Cerny AM, Zacharia A, Fitzgerald KA, Kurt-Jones EA, Finberg RW. Induction and inhibition of type I interferon responses by distinct components of lymphocytic choriomeningitis virus. J Virol 84(18) 9452-62(2010) 20592086 Abe T, Kaname Y, Wen X, Tani H, Moriishi K, Uematsu S, Takeuchi O, Ishii KJ, Kawai T, Akira S, Matsuura Y. Baculovirus induces type I interferon production through toll-like receptor-dependent and -independent pathways in a cell-type-specific manner. J Virol 83(15) 7629-40(2009) 19474102 Johnson J, Molle C, Aksoy E, Goldman M, Goriely S, Willems F. A conventional protein kinase C inhibitor targeting IRF-3-dependent genes differentially regulates IL-12 family members. Mol Immunol 48(12-13) 1484-93(2011) 21550664 Warzée B, Mesnil C, Hober D, Caloone D, Pirottin D, Garigliany MM, Desmecht D, Drion PV, Journot L, Lekeux P, Bureau F, Desmet CJ. Viral induction of Zac1b through TLR3- and IRF3-dependent pathways. Mol Immunol 48(1-3) 119-27(2010) 20947170 Ines Ambite, Thi Hien Tran, Daniel S. C. Butler, Michele Cavalera, Murphy Lam Yim Wan, Shahram Ahmadi, Catharina Svanborg Therapeutic Effects of IL-1RA against Acute Bacterial Infections, including Antibiotic-Resistant Strains Pathogens 13 42(2023) 38251349 Sakaguchi T, Kato A, Kiyotani K, Yoshida T, Nagai Y. Studies on the paramyxovirus accessory genes by reverse genetics in the Sendai virus-mouse system. Proc Jpn Acad Ser B Phys Biol Sci 84(10) 439-51(2008) 19075516 Ysebrant de Lendonck L, Tonon S, Nguyen M, Vandevenne P, Welsby I, Martinet V, Molle C, Charbonnier LM, Leo O, Goriely S. Interferon regulatory factor 3 controls interleukin-17 expression in CD8 T lymphocytes. Proc Natl Acad Sci U S A 110(34) E3189-97(2013) 23918362 Kiyotani K, Sakaguchi T, Kato A, Nagai Y, Yoshida T. Paramyxovirus Sendai virus V protein counteracts innate virus clearance through IRF-3 activation, but not via interferon, in mice. Virology 359(1) 82-91(2007) 17027894

BRC mice in Publications

Foss MH, Stevens SL, Jin H, Allen EM, Nelson D, DeFilippis V, Nilsen A, Stenzel-Poore MP.
Identification and Characterization of Small-Molecule IRF3-Dependent Immune Activators for Pharmaceutical Development.
ACS Chem Biol 17(5) 1073-1081(2022) 35471821

Smith H, Liu XY, Dai L, Goh ET, Chan AT, Xi J, Seh CC, Qureshi IA, Lescar J, Ruedl C, Gourlay R, Morton S, Hough J, McIver EG, Cohen P, Cheung PC.
The role of TBK1 and IKKε in the expression and activation of Pellino 1.
Biochem J 434(3) 537-48(2011) 21204785

Goriely S, Molle C, Nguyen M, Albarani V, Haddou NO, Lin R, De Wit D, Flamand V, Willems F, Goldman M.
Interferon regulatory factor 3 is involved in Toll-like receptor 4 (TLR4)- and TLR3-induced IL-12p35 gene activation.
Blood 107(3) 1078-84(2006) 16219795

Zhang SM, Zhu LH, Li ZZ, Wang PX, Chen HZ, Guan HJ, Jiang DS, Chen K, Zhang XF, Tian S, Yang D, Zhang XD, Li H.
Interferon regulatory factor 3 protects against adverse neo-intima formation.
Cardiovasc Res 102(3) 469-79(2014) 24596398

Benmerzoug S, Bounab B, Rose S, Gosset D, Biet F, Cochard T, Xavier A, Rouxel N, Fauconnier L, Horsnell WGC, Ryffel B, Togbe D, Quesniaux VFJ.
Sterile Lung Inflammation Induced by Silica Exacerbates Mycobacterium tuberculosis Infection via STING-Dependent Type 2 Immunity.
Cell Rep 27(9) 2649-2664.e5(2019) 31141689

Dogusan Z, García M, Flamez D, Alexopoulou L, Goldman M, Gysemans C, Mathieu C, Libert C, Eizirik DL, Rasschaert J.
Double-stranded RNA induces pancreatic beta-cell apoptosis by activation of the toll-like receptor 3 and interferon regulatory factor 3 pathways.
Diabetes 57(5) 1236-45(2008) 18223009

Sin WX, Yeong JP, Lim TJF, Su IH, Connolly JE, Chin KC.
IRF-7 Mediates Type I IFN Responses in Endotoxin-Challenged Mice.
Front Immunol 11 640(2020) 32373120

Loi P, Yuan Q, Torres D, Delbauve S, Laute MA, Lalmand MC, Pétein M, Goriely S, Goldman M, Flamand V.
Interferon regulatory factor 3 deficiency leads to interleukin-17-mediated liver ischemia-reperfusion injury.
Hepatology 57(1) 351-61(2013) 22911673

Prantner D, Sikes JD, Hennings L, Savenka AV, Basnakian AG, Nagarajan UM.
Interferon regulatory transcription factor 3 protects mice from uterine horn pathology during Chlamydia muridarum genital infection.
Infect Immun 79(10) 3922-33(2011) 21788382

Marichal T, Bedoret D, Mesnil C, Pichavant M, Goriely S, Trottein F, Cataldo D, Goldman M, Lekeux P, Bureau F, Desmet CJ.
Interferon response factor 3 is essential for house dust mite-induced airway allergy.
J Allergy Clin Immunol 126(4) 836-844.e13(2010) 20673978

Ishii T, Hosoki K, Nikura Y, Yamashita N, Nagase T, Yamashita N.
IFN Regulatory Factor 3 Potentiates Emphysematous Aggravation by Lipopolysaccharide.
J Immunol 198(9) 3637-3649(2017) 28363903

Prantner D, Darville T, Nagarajan UM.
Stimulator of IFN gene is critical for induction of IFN-beta during Chlamydia muridarum infection.
J Immunol 184(5) 2551-60(2010) 20107183

Molle C, Goldman M, Goriely S.
Critical role of the IFN-stimulated gene factor 3 complex in TLR-mediated IL-27p28 gene expression revealing a two-step activation process.
J Immunol 184(4) 1784-92(2010) 20083668

Molle C, Nguyen M, Flamand V, Renneson J, Trottein F, De Wit D, Willems F, Goldman M, Goriely S.
IL-27 synthesis induced by TLR ligation critically depends on IFN regulatory factor 3.
J Immunol 178(12) 7607-15(2007) 17548596

Liu Y, Mo CF, Luo XY, Li H, Guo HJ, Sun H, Hu S, Li LM, Wang YT, Yang SX, Chang S, Zou Q.
Activation of Toll-Like Receptor 3 Induces Interleukin-1 Receptor Antagonist Expression by Activating the Interferon Regulatory Factor 3.
J Innate Immun 12(4) 304-320(2020) 31865314

Stevens SL, Leung PY, Vartanian KB, Gopalan B, Yang T, Simon RP, Stenzel-Poore MP.
Multiple preconditioning paradigms converge on interferon regulatory factor-dependent signaling to promote tolerance to ischemic brain injury.
J Neurosci 31(23) 8456-63(2011) 21653850

Marsh B, Stevens SL, Packard AE, Gopalan B, Hunter B, Leung PY, Harrington CA, Stenzel-Poore MP.
Systemic lipopolysaccharide protects the brain from ischemic injury by reprogramming the response of the brain to stroke: a critical role for IRF3.
J Neurosci 29(31) 9839-49(2009) 19657036

Christensen JE, Fenger C, Issazadeh-Navikas S, Krug A, Liljestrøm P, Goriely S, Paludan SR, Finsen B, Christensen JP, Thomsen AR.
Differential impact of interferon regulatory factor 7 in initiation of the type I interferon response in the lymphocytic choriomeningitis virus-infected central nervous system versus the periphery.
J Virol 86(13) 7384-92(2012) 22514347

Zhou S, Cerny AM, Zacharia A, Fitzgerald KA, Kurt-Jones EA, Finberg RW.
Induction and inhibition of type I interferon responses by distinct components of lymphocytic choriomeningitis virus.
J Virol 84(18) 9452-62(2010) 20592086

Abe T, Kaname Y, Wen X, Tani H, Moriishi K, Uematsu S, Takeuchi O, Ishii KJ, Kawai T, Akira S, Matsuura Y.
Baculovirus induces type I interferon production through toll-like receptor-dependent and -independent pathways in a cell-type-specific manner.
J Virol 83(15) 7629-40(2009) 19474102

Johnson J, Molle C, Aksoy E, Goldman M, Goriely S, Willems F.
A conventional protein kinase C inhibitor targeting IRF-3-dependent genes differentially regulates IL-12 family members.
Mol Immunol 48(12-13) 1484-93(2011) 21550664

Warzée B, Mesnil C, Hober D, Caloone D, Pirottin D, Garigliany MM, Desmecht D, Drion PV, Journot L, Lekeux P, Bureau F, Desmet CJ.
Viral induction of Zac1b through TLR3- and IRF3-dependent pathways.
Mol Immunol 48(1-3) 119-27(2010) 20947170

Ines Ambite, Thi Hien Tran, Daniel S. C. Butler, Michele Cavalera, Murphy Lam Yim Wan, Shahram Ahmadi, Catharina Svanborg
Therapeutic Effects of IL-1RA against Acute Bacterial Infections, including Antibiotic-Resistant Strains
Pathogens 13 42(2023) 38251349

Sakaguchi T, Kato A, Kiyotani K, Yoshida T, Nagai Y.
Studies on the paramyxovirus accessory genes by reverse genetics in the Sendai virus-mouse system.
Proc Jpn Acad Ser B Phys Biol Sci 84(10) 439-51(2008) 19075516

Ysebrant de Lendonck L, Tonon S, Nguyen M, Vandevenne P, Welsby I, Martinet V, Molle C, Charbonnier LM, Leo O, Goriely S.
Interferon regulatory factor 3 controls interleukin-17 expression in CD8 T lymphocytes.
Proc Natl Acad Sci U S A 110(34) E3189-97(2013) 23918362

Kiyotani K, Sakaguchi T, Kato A, Nagai Y, Yoshida T.
Paramyxovirus Sendai virus V protein counteracts innate virus clearance through IRF-3 activation, but not via interferon, in mice.
Virology 359(1) 82-91(2007) 17027894

Strain Information

Health Report

Gene

Ordering Information

BRC mice in Publications