Strain Information | |
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Image | |
BRC No. | RBRC00850 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | B6.129P2-Itgal<tm1Mak> |
Former Common name | C57BL/6-[KO]LFA-1; B6.129P2-Itgb2<tm1Oci> |
H-2 Haplotype | |
ES Cell line | E14K [129P2/OlaHsd] |
Background strain | |
Appearance | black |
Strain development | From Tak. W. Mak of ontario Cancer Institute, N6 |
Strain description | Original line was established by Tak Mak at Ontario Cancer Institute in Tronto (Schmits et al., J. Exp. Med., 183, 1415, 1996). Neo registance gene construct was introduced into the CD11a allele of an ES cell line E14k of 129/Ola origin. Mutant ES cells were injected to C57BL/6 blastocysts. Chimeras were mated with C57BL/6 and was backcrossed to C57BL/6 for 6 generations. After embryo transfer at Taconic, the colony has been maintained through intercrossing of homozygous mice. |
Colony maintenance | |
References | J. Exp. Med., 183, 1415-1426 (1996). 8666900 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)7neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter) |
Ordering Information | |
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供与核酸 | herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, tk poly A, mouse CD11a genomic DNA |
Research application | Immunology and Inflammation Research Virology Research covid19_infection |
提供条件 | 条件を付加する。 利用者はnon-profit organizationを対象とし、Dr. Tak W. Makの許可を得ること。 開発者連絡先:Tak W. Mak, Division of Stem Cell and Developmental Biology, Advanced Medical Discovery Institute/Ontario Cancer Institute E-mail: tmak@uhnresearch.ca |
Depositor | 小安 重夫(慶應義塾大学) |
Strain Status | 凍結胚 |
Strain Availability | 凍結胚より作出したマウスを2~4ヶ月以内に提供可能 凍結胚を1ヶ月以内に提供可能 |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Saitoh SI, Abe F, Kanno A, Tanimura N, Mori Saitoh Y, Fukui R, Shibata T, Sato K, Ichinohe T, Hayashi M, Kubota K, Kozuka-Hata H, Oyama M, Kikko Y, Katada T, Kontani K, Miyake K. TLR7 mediated viral recognition results in focal type I interferon secretion by dendritic cells. Nat Commun 8(1) 1592(2017) 29150602 |