Strain Data Sheet

RBRC00843

Strain Information

Image
BRC No.RBRC00843
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Maf<tm1Mym>/2Rbrc
Former Common name44A, c-Maf/2 KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6J
Appearance
Strain developmentDeveloped by Drs. Shimako Kawauchi and Satoru Takahashi at University of Tsukuba in 1998. Both the neomycin and lacZ gene were inserted and replacing most of the coding region of c-Maf gene.
Strain descriptionB6;129P2-Maf<tm2Mym>. c-Maf is a transcriptional factor that belongs to the large Maf family. This gene is essential for normal lens development. Homozygous mutant mice show defective differentiation of lens fiber cells, and the expression of crystalin genes was severely impaired in the mice.
Colony maintenanceSibling Mating (Heterozygote x Wild-type)Homozygous mutant mice die within 3 days after birth.
References
Regulation of lens fiber cell differentiation by transcription factor c-Maf.
Kawauchi S, Takahashi S, Nakajima O, Ogino H, Morita M, Nishizawa M, Yasuda K, Yamamoto M
J. Biol. Chem., 274, 19254-19260 (1999). 10383433

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Maf
MGI:96909		MAF bZIP transcription factor8Maf<tm1Mym>
MGI:2148637		targeted mutation 1, Masayuki Yamamoto
  • Ayme-Gripp syndrome(MedGEN)

  • cataract 21 multiple types(MedGEN)
    • lacZ beta-galactosidase (E. coli)8
    neo neomycin resistance gene (E. coli)8 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal lens development(MP:0005545)

  • abnormal lens fiber morphology(MP:0002840)

  • embryonic lethality during organogenesis, incomplete penetrance(MP:0011108)

  • postnatal lethality, complete penetrance(MP:0011085)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse c-Maf genomic DNA, E. coli neomycin phosphotransferase, E. coli lacZ, herpes simplex virus thymidine kinase promoter (HSV tk promoter)
    Research applicationImmunology and Inflammation Research
    Sensorineural Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
    DepositorSatoru Takahashi (University of Tsukuba)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data