Strain Data Sheet

RBRC00824

Strain Information

Image
BRC No.RBRC00824
TypeChromosomal Aberration
SpeciesMus musculus
Strain nameSTOCK T(X;16)16H +/+ Eda<Ta>/Rbrc
Former Common nameSearle 転座, T(X;16)16H
H-2 Haplotype
ES Cell line
Background strain
Appearancewild [T16H/+]
Tabby [XY]
Strain development1964年、Anthony Searle、MRC Radiobiology Unit, Harwell, UK. Genetic Variants and Strains of the Laboratory Mouse, 2, 1459参照。(C3H♀×101♂)×Ta/Y 前核期にX線照射して得られたsemi-sterile雌が起源。この雌はTa/+でありながら、表現型は野生型となる。原因は正常X染色体が体細胞で不活性化しているため。
Strain descriptionXと16番染色体との相互転座。不活性化センターが16xにあるため、X16は不活性化出来ない。16xが不活性化すると遺伝的にアンバランスが生じ、淘汰される。結局正常X染色体が不活性化した細胞が生き残り、ヘテロ接合体でも表現型はホモ接合体と同じになる。巾広く利用されている。
Colony maintenance雄は不妊の為、semi-sterileの雌で維持する必要がある。通常野生型の雌にTaの雄を交配し、wild-typeの雌とTa雄のみを残し、交配を継ける。wild-typeの雄はcarrierで不妊。Taの雌はX/Xである。着床数は正常雌の半数以下、受精後、7.5日目でほぼ半数が消失している。従って、なるべく多くの雌を飼う必要がある。
References
Reversal of X-inactivation in female mouse somatic cells hybridized with murine teratocarcinoma stem cells in vitro.
Takagi N, Yoshida M A, Sugawara O, Sasaki M
Cell, 34, 1053-1062 (1983). 6627391

Cytogenetics of the Mammalian X Chromosome, Part A Basic Mechanisms of X Chromosome Behavior, 21-50 (1983).

Detrimental effects of two active X chromosomes on early mouse development.
Takagi N, Abe K
Development, 109, 189-201 (1990). 2209464

Translocation breakpoint possibly predisposes to nonrandom X-chromosome inactivation in mouse embryos bearing Searle's T(X;16)16H translocation.
Ogura H, Takada S, Mise N, Sugimoto M, Tan S S, Takagi N
Cytogenet. Cell Genet., 80, 173-178 (1998). 9678353

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
T(X;16)16H
MGI:104279		reciprocal translocation, Chr X and 16, Harwell 1616T(X;16)16H
MGI:3032980
Eda
MGI:1195272		ectodysplasin-AXEda<Ta> tabby
  • tooth agenesis, selective, X-linked, 1(MedGEN)

  • X-linked hypohidrotic ectodermal dysplasia(DisGeNET, MedGEN)
    • T(X;16)16H
    MGI:104278    		reciprocal translocation, Chr X and 16, Harwell 16XT(X;16)16H
    MGI:3032981

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal autopod morphology (MP:0000572)

  • abnormal coat appearance (MP:0001510)

  • abnormal coat/ hair morphology (MP:0000367)

  • abnormal coat/hair pigmentation (MP:0002075)

  • abnormal enamel morphology (MP:0002576)
    • more 40 phenotypes
  • abnormal eye morphology (MP:0002092)

  • abnormal eyelid morphology (MP:0001340)

  • abnormal guard hair morphology (MP:0000397)

  • abnormal hair follicle development (MP:0003704)

  • abnormal hair follicle melanocyte morphology (MP:0004381)

  • abnormal hair follicle pheomelanosome pheomelanin content (MP:0006370)

  • abnormal hair growth (MP:0002073)

  • abnormal hair texture (MP:0002074)

  • abnormal incisor morphology (MP:0005358)

  • abnormal lateral nasal gland morphology (MP:0013582)

  • abnormal molar morphology (MP:0002689)

  • abnormal molar root morphology (MP:0030494)

  • abnormal nasal cavity morphology (MP:0002237)

  • abnormal nasal gland morphology (MP:0013580)

  • abnormal nose morphology (MP:0002233)

  • abnormal palatal rugae morphology (MP:0009652)

  • abnormal respiratory mucosa morphology (MP:0002277)

  • abnormal respiratory system morphology (MP:0002132)

  • abnormal skeleton morphology (MP:0005508)

  • abnormal skin pigmentation (MP:0002095)

  • abnormal tail morphology (MP:0002111)

  • abnormal tail ring morphology (MP:0012078)

  • abnormal tail tip morphology (MP:0013177)

  • abnormal vibrissa number (MP:0009003)

  • absent Meibomian glands (MP:0006236)

  • absent eccrine glands (MP:0000577)

  • absent guard hair (MP:0006365)

  • absent hair follicle pheomelanosome pheomelanin (MP:0006371)

  • absent vibrissae (MP:0001284)

  • absent zigzag hairs (MP:0006366)

  • focal hair loss (MP:0000418)

  • hairless tail (MP:0010233)

  • irregular coat pigmentation (MP:0000372)

  • kinked tail (MP:0000585)

  • narrow eye opening (MP:0005287)

  • short incisors (MP:0004830)

  • striated fur (MP:0009659)

  • thin epidermis (MP:0001218)

  • thin skin (MP:0001199)

  • transverse fur striping (MP:0010231)
    • Detailed phenotype data

    Ordering Information

    供与核酸
    Research applicationDevelopmental Biology Research
    提供条件条件を付加しない。
    Depositor高木　信夫（北海道大学）
    Strain Status凍結胚のアイコン凍結胚
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)

    BRC mice in Publications

    No Data