Strain Data Sheet

RBRC00821

update : 2024-04-19

Strain Information
Image
BRC No.	RBRC00821
Type	Transgene
Species	Mus musculus
Strain name	C57BL/6J-Tg(deGFP)18Imeg
Former Common name	Oct-delta PE-GFP #18, TgN(deGFP)18Imeg
H-2 Haplotype
ES Cell line
Background strain	C57BL/6JJcl
Appearance	black
Strain development	理化学研究所バイオリソースセンター（熊本大学発生医学研究センター）・阿部訓也先生（1999）。C57BL/6J受精卵にTgのインジェクションにより作出。ライン18（RBRC00821）、ライン20（RBRC00822）。
Strain description	POU転写因子Oct-4 (Pou5f1) 遺伝子を含む18kbのゲノム断片 (Genomic Oct-4 Fragment of 18) とGFP遺伝子との融合遺伝子を導入したトランスジェニックマウス。GOF18のproximal enhancer (PE) 部は除去されている。始原生殖細胞・未分化全能性細胞特異的にGFPを発現する。
Colony maintenance	Carrier x Noncarrier [C57BL/6JJcl]
References	Dev. Biol., 258, 209-225 (2003). 12781694

Health Report
Examination Date / Room / Rack

Gene
Gene info	Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GFPGreen Fluorescent Protein (Aequorea victoria)UNmouse Oct 3/4 promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyAUN

Ordering Information
供与核酸	mouse Oct 3/4 genomic DNA, Jellyfish GFP cDNA, SV40 polyA
Research application	Developmental Biology Research Fluorescent Proteins/lacZ System Reproductive Biology Research
提供条件	条件を付加する。利用者は事前に寄託者の提供承諾書を得る。研究成果の公表にあたって寄託者の指定する文献を引用する。Identification and characterization of stem cells in prepubertal spermatogenesis in mice. Dev. Biol., 258, 209-225 (2003). 寄託後１年間は、共著を必要とする共同研究とする。当該リソースを使用した研究成果に基づき特許等の申請を行う場合は、別途協議を行う。
Depositor	阿部　訓也（理化学研究所筑波研究所）
Strain Status	凍結胚凍結精子
Strain Availability	凍結胚より作出したマウスを2~4ヶ月以内に提供可能凍結精子を1ヶ月以内に提供可能凍結胚を1ヶ月以内に提供可能
Additional Info.	Necessary documents for ordering: Approval form (Japanese / English) Order form (Japanese / English) Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English) Acceptance of responsibility for living modified organism (Japanese / English) Lab HP Genotyping protocol -PCR- Mouse of the Month Sep 2005

BRC mice in Publications

Sugimoto M, Kondo M, Hirose M, Suzuki M, Mekada K, Abe T, Kiyonari H, Ogura A, Takagi N, Artzt K, Abe K. Molecular identification of t(w5): Vps52 promotes pluripotential cell differentiation through cell-cell interactions. Cell Rep 2(5) 1363-74(2012) 23142660 Ikeda R, Shiura H, Numata K, Sugimoto M, Kondo M, Mise N, Suzuki M, Greally JM, Abe K. Large, male germ cell-specific hypomethylated DNA domains with unique genomic and epigenomic features on the mouse X chromosome. DNA Res 20(6) 549-65(2013) 23861320 Mise N, Fuchikami T, Sugimoto M, Kobayakawa S, Ike F, Ogawa T, Tada T, Kanaya S, Noce T, Abe K. Differences and similarities in the developmental status of embryo-derived stem cells and primordial germ cells revealed by global expression profiling. Genes Cells 13(8) 863-77(2008) 18782224 Miki H, Inoue K, Kohda T, Honda A, Ogonuki N, Yuzuriha M, Mise N, Matsui Y, Baba T, Abe K, Ishino F, Ogura A. Birth of mice produced by germ cell nuclear transfer. Genesis 41(2) 81-6(2005) 15712265 Cao L, Shitara H, Sugimoto M, Hayashi J, Abe K, Yonekawa H. New evidence confirms that the mitochondrial bottleneck is generated without reduction of mitochondrial DNA content in early primordial germ cells of mice. PLoS Genet 5(12) e1000756(2009) 19997484 Takehara T, Teramura T, Onodera Y, Hamanishi C, Fukuda K. Reduced oxygen concentration enhances conversion of embryonic stem cells to epiblast stem cells. Stem Cells Dev 21(8) 1239-49(2012) 21861689

BRC mice in Publications

Sugimoto M, Kondo M, Hirose M, Suzuki M, Mekada K, Abe T, Kiyonari H, Ogura A, Takagi N, Artzt K, Abe K.
Molecular identification of t(w5): Vps52 promotes pluripotential cell differentiation through cell-cell interactions.
Cell Rep 2(5) 1363-74(2012) 23142660

Ikeda R, Shiura H, Numata K, Sugimoto M, Kondo M, Mise N, Suzuki M, Greally JM, Abe K.
Large, male germ cell-specific hypomethylated DNA domains with unique genomic and epigenomic features on the mouse X chromosome.
DNA Res 20(6) 549-65(2013) 23861320

Mise N, Fuchikami T, Sugimoto M, Kobayakawa S, Ike F, Ogawa T, Tada T, Kanaya S, Noce T, Abe K.
Differences and similarities in the developmental status of embryo-derived stem cells and primordial germ cells revealed by global expression profiling.
Genes Cells 13(8) 863-77(2008) 18782224

Miki H, Inoue K, Kohda T, Honda A, Ogonuki N, Yuzuriha M, Mise N, Matsui Y, Baba T, Abe K, Ishino F, Ogura A.
Birth of mice produced by germ cell nuclear transfer.
Genesis 41(2) 81-6(2005) 15712265

Cao L, Shitara H, Sugimoto M, Hayashi J, Abe K, Yonekawa H.
New evidence confirms that the mitochondrial bottleneck is generated without reduction of mitochondrial DNA content in early primordial germ cells of mice.
PLoS Genet 5(12) e1000756(2009) 19997484

Takehara T, Teramura T, Onodera Y, Hamanishi C, Fukuda K.
Reduced oxygen concentration enhances conversion of embryonic stem cells to epiblast stem cells.
Stem Cells Dev 21(8) 1239-49(2012) 21861689

Strain Information

Health Report

Gene

Ordering Information

BRC mice in Publications