Strain Data Sheet

RBRC00821

Strain Information

Image
BRC No.RBRC00821
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(deGFP)18Imeg
Former Common nameOct-delta PE-GFP #18, TgN(deGFP)18Imeg
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack
Strain development理化学研究所バイオリソースセンター（熊本大学発生医学研究センター）・阿部訓也先生（1999）。C57BL/6J受精卵にTgのインジェクションにより作出。ライン18（RBRC00821）、ライン20（RBRC00822）。
Strain descriptionPOU転写因子Oct-4 (Pou5f1) 遺伝子を含む18kbのゲノム断片 (Genomic Oct-4 Fragment of 18) とGFP遺伝子との融合遺伝子を導入したトランスジェニックマウス。GOF18のproximal enhancer (PE) 部は除去されている。始原生殖細胞・未分化全能性細胞特異的にGFPを発現する。
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References
Identification and characterization of stem cells in prepubertal spermatogenesis in mice.
Ohbo K, Yoshida S, Ohmura M, Ohneda O, Ogawa T, Tsuchiya H, Kuwana T, Kehler J, Abe K, Scholer H R, Suda T
Dev. Biol., 258, 209-225 (2003). 12781694

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyAUN
GFPGreen Fluorescent Protein (Aequorea victoria)UNmouse Oct 3/4 promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    供与核酸mouse Oct 3/4 genomic DNA, Jellyfish GFP cDNA, SV40 polyA
    Research applicationDevelopmental Biology Research
    Fluorescent Proteins/lacZ System
    Reproductive Biology Research
    提供条件利用者は事前に寄託者の提供承諾書を得る。研究成果の公表にあたって寄託者の指定する文献を引用する。Dev. Biol., 258, 209-225 (2003). 寄託後１年間は、共著を必要とする共同研究とする。当該リソースを使用した研究成果に基づき特許等の申請を行う場合は、別途協議を行う。
    Depositor阿部　訓也（理化学研究所筑波研究所）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-
    Mouse of the Month Sep 2005

    BRC mice in Publications

    Sugimoto M, Kondo M, Hirose M, Suzuki M, Mekada K, Abe T, Kiyonari H, Ogura A, Takagi N, Artzt K, Abe K.
    Molecular identification of t(w5): Vps52 promotes pluripotential cell differentiation through cell-cell interactions.
    Cell Rep 2(5) 1363-74(2012) 23142660
    Ikeda R, Shiura H, Numata K, Sugimoto M, Kondo M, Mise N, Suzuki M, Greally JM, Abe K.
    Large, male germ cell-specific hypomethylated DNA domains with unique genomic and epigenomic features on the mouse X chromosome.
    DNA Res 20(6) 549-65(2013) 23861320
    Mise N, Fuchikami T, Sugimoto M, Kobayakawa S, Ike F, Ogawa T, Tada T, Kanaya S, Noce T, Abe K.
    Differences and similarities in the developmental status of embryo-derived stem cells and primordial germ cells revealed by global expression profiling.
    Genes Cells 13(8) 863-77(2008) 18782224
    Miki H, Inoue K, Kohda T, Honda A, Ogonuki N, Yuzuriha M, Mise N, Matsui Y, Baba T, Abe K, Ishino F, Ogura A.
    Birth of mice produced by germ cell nuclear transfer.
    Genesis 41(2) 81-6(2005) 15712265
    Cao L, Shitara H, Sugimoto M, Hayashi J, Abe K, Yonekawa H.
    New evidence confirms that the mitochondrial bottleneck is generated without reduction of mitochondrial DNA content in early primordial germ cells of mice.
    PLoS Genet 5(12) e1000756(2009) 19997484
    Takehara T, Teramura T, Onodera Y, Hamanishi C, Fukuda K.
    Reduced oxygen concentration enhances conversion of embryonic stem cells to epiblast stem cells.
    Stem Cells Dev 21(8) 1239-49(2012) 21861689