Strain Data Sheet

RBRC00820

Strain Information

Image
BRC No.RBRC00820
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Qk<tm1Imeg>/Rbrc
Former Common nameqkI Knockout, qkI遺伝子のノックアウトマウス, TgH(qkI)2Imeg
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearanceagouti
Strain development熊本大学発生医学研究センター・阿部訓也・山村研一。E14.1 ES細胞を用いて作出。
Strain descriptionQk (quaking) 遺伝子のノックアウトマウス。Qk遺伝子の開始コドンとQUA1ドメインの保存性の高い最初の40アミノ酸領域をIRES-betageo遺伝子で置換。ホモマウスは胎生期9.5〜10.5日に致死となる。
Colony maintenanceHeterozygote × Wild-type [C57BL/6JJcl]
References
Defective smooth muscle development in qkI-deficient mice.
Zhenghua Li, Nobuyuki Takakura, Yuichi Oike, Takanobu Imanaka, Kimi Araki, Toshio Suda, Tadashi Kaname, Tatsuya Kondo, Kuniya Abe, Ken-Ichi Yamamura
Dev. Growth Differ., 45, 449-462 (2003). 14706070

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Qk
MGI:97837		quaking17Qk<tm1Imeg>
MGI:3033861		targeted mutation 1, Institute of Molecular Embryology and Genetics
lacZ beta-galactosidase (E. coli)17 promoter less
neo neomycin resistance gene (E. coli)17
SV40 polyAUN

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal blood vessel morphology (MP:0001614)

  • abnormal heart morphology (MP:0000266)

  • abnormal hematopoietic system morphology/development (MP:0002396)

  • abnormal myocardium layer morphology (MP:0005329)

  • abnormal neural tube morphology (MP:0002151)
    • more 8 phenotypes
  • abnormal visceral yolk sac morphology (MP:0001718)

  • abnormal vitelline vasculature morphology (MP:0003229)

  • decreased embryo size (MP:0001698)

  • embryonic lethality during organogenesis, complete penetrance (MP:0011098)

  • incomplete embryo turning (MP:0001701)

  • open neural tube (MP:0000929)

  • pericardial effusion (MP:0005312)

  • wavy neural tube (MP:0000930)
    • Detailed phenotype data

    Ordering Information

    供与核酸Encephalomyocarditis virus (EMCV) internal ribosomal entry site (IRES), beta-geo(E. coli lacZ, E. coli neo), SV40 poly A, mouse qkI genomic DNA
    Research applicationFluorescent Proteins/lacZ System
    提供条件利用者は事前に寄託者の提供承諾書を得る。研究成果の公表にあたって寄託者の指定する文献を引用する。Dev. Growth Differ., 45, 449-462 (2003). 寄託後１年間は、共著を必要とする共同研究とする。当該リソースを使用した研究成果に基づき特許等の申請を行う場合は、別途協議を行う。
    Depositor阿部　訓也（理化学研究所筑波研究所）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data