Strain Data Sheet

RBRC00820

Strain Information

Image
BRC No.RBRC00820
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Qk<tm1Imeg>/Rbrc
Former Common nameqkI Knockout mouse, TgH(qkI)2Imeg
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearanceagouti
Strain developmentDeveloped by Kuniya Abe and Ken-ichi Yamamura, Insitute of Molecular Embryology and Genetics, Kumamoto University. E14.1 ES cells were used for generation of the knockout mice. The mutant mice were crossed to C57BL/6J.
Strain descriptionQk (quaking) gene knockout mice. The initiation codon and first 40 amino acids of a highly conserved QUA1 domain of Qk gene were replaced with an IRES and a betageo fusion gene. Homozygous mutant mice die in utero E9.5 and E10.5.
Colony maintenanceHeterozygote × Wild-type [C57BL/6JJcl]
References
Defective smooth muscle development in qkI-deficient mice.
Zhenghua Li, Nobuyuki Takakura, Yuichi Oike, Takanobu Imanaka, Kimi Araki, Toshio Suda, Tadashi Kaname, Tatsuya Kondo, Kuniya Abe, Ken-Ichi Yamamura
Dev. Growth Differ., 45, 449-462 (2003). 14706070

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Qk
MGI:97837		quaking17Qk<tm1Imeg>
MGI:3033861		targeted mutation 1, Institute of Molecular Embryology and Genetics
lacZ beta-galactosidase (E. coli)17 promoter less
neo neomycin resistance gene (E. coli)17
SV40 polyAUN

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal blood vessel morphology (MP:0001614)

  • abnormal heart morphology (MP:0000266)

  • abnormal hematopoietic system morphology/development (MP:0002396)

  • abnormal myocardium layer morphology (MP:0005329)

  • abnormal neural tube morphology (MP:0002151)
    • more 8 phenotypes
  • abnormal visceral yolk sac morphology (MP:0001718)

  • abnormal vitelline vasculature morphology (MP:0003229)

  • decreased embryo size (MP:0001698)

  • embryonic lethality during organogenesis, complete penetrance (MP:0011098)

  • incomplete embryo turning (MP:0001701)

  • open neural tube (MP:0000929)

  • pericardial effusion (MP:0005312)

  • wavy neural tube (MP:0000930)
    • Detailed phenotype data

    Ordering Information

    Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (IRES), beta-geo(E. coli lacZ, E. coli neo), SV40 poly A, mouse qkI genomic DNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Growth Differ., 45, 449-462 (2003). The DEPOSITOR should be a co-author in articles when the users publish their data using these mice for 1 year after provided to the RIKEN BRC.
    Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice.
    DepositorKuniya Abe (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data