Strain Data Sheet
RBRC00808
Strain Information | |
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| Image | |
| BRC No. | RBRC00808 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.129P2-Emx1<tm1(cre)Ito>/ItoRbrc |
| Former Common name | Emx1-Cre |
| H-2 Haplotype | |
| ES Cell line | E14 [129P2/OlaHsd] |
| Background strain | C57BL/6JJcl |
| Appearance | black (a/a B/B C/C) |
| Strain development | 理化学研究所脳科学総合研究センター・岩里琢治、糸原重美。E14 ES細胞を用いて作出。C57BL/6背景。 |
| Strain description | Emx1-creノックインマウス。Cre遺伝子とPGK-neoカセットがEmx1遺伝子の翻訳開始コドンATGの前に挿入されているマウス (neo型) (RBRC00808) とPGK-neoカセットを除去したマウス (delta neo型) (RBRC01345) 。大脳皮質、海馬、嗅球の興奮性神経細胞特異的にCre組換え酵素を発現する。delta neo型マウス (RBRC01345) では、neo型で観察されたectopic expressionが消失しており、内在性遺伝子の発現特性をより反映している。ホモマウスは生存性、繁殖性ともにあり。 |
| Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
| References | Nature, 406, 726-731 (2000). 10963597 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Emx1empty spiracles homolog 1 (Drosophila)6Emx1<tm1(cre)Ito>targeted mutation 1 (cre), Shigeyoshi Itohara Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter crePhage P1 Cre recombinase6cremouse Emx1 (Drosophila homeobox gene empty spiracles (Ems) mouse homolog) promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)6neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter nlsSV40 large T antigen nuclear localization signal (NLS)6nls Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA6 |
Ordering Information | |
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| 供与核酸 | Simian virus 40 Large T antigen nuclear localization signal (NLS), phage P1 Cre recombinase, SV40 poly A, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Emx1 (Drosophila homeobox gene empty spiracles (Ems) mouse homolog) genomic DNA |
| Research application | Cell Biology Research Cre/loxP system FLP/frt system Neurobiology Research |
| 提供条件 | 条件を付加する。 研究成果の公表にあたって寄託者の指定する文献を引用する。Nature, 406, 726-731 (2000). |
| Depositor | 糸原 重美（理化学研究所脳科学総合研究センター） |
| Strain Status |  凍結胚 凍結精子 |
| Strain Availability | 凍結胚より作出したマウスを2~4ヶ月以内に提供可能 凍結精子を1ヶ月以内に提供可能 凍結胚を1ヶ月以内に提供可能 |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month June 2006 |
BRC mice in Publications |
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Imayoshi I, Shimogori T, Ohtsuka T, Kageyama R. Hes genes and neurogenin regulate non-neural versus neural fate specification in the dorsal telencephalic midline. Development 135(15) 2531-41(2008) 18579678 Kita A, Imayoshi I, Hojo M, Kitagawa M, Kokubu H, Ohsawa R, Ohtsuka T, Kageyama R, Hashimoto N. Hes1 and Hes5 control the progenitor pool, intermediate lobe specification, and posterior lobe formation in the pituitary development. Mol Endocrinol 21(6) 1458-66(2007) 17426285 Kinameri E, Inoue T, Aruga J, Imayoshi I, Kageyama R, Shimogori T, Moore AW. Prdm proto-oncogene transcription factor family expression and interaction with the Notch-Hes pathway in mouse neurogenesis. PLoS One 3(12) e3859(2008) 19050759 |