Strain Data Sheet

RBRC00808

Strain Information

Image
BRC No.RBRC00808
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Emx1<tm1(cre)Ito>/ItoRbrc
Former Common nameEmx1-Cre
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearanceblack (a/a B/B C/C)
Strain development理化学研究所脳科学総合研究センター・岩里琢治、糸原重美。E14 ES細胞を用いて作出。C57BL/6背景。
Strain descriptionEmx1-creノックインマウス。Cre遺伝子とPGK-neoカセットがEmx1遺伝子の翻訳開始コドンATGの前に挿入されているマウス (neo型) (RBRC00808) とPGK-neoカセットを除去したマウス (delta neo型) (RBRC01345) 。大脳皮質、海馬、嗅球の興奮性神経細胞特異的にCre組換え酵素を発現する。delta neo型マウス (RBRC01345) では、neo型で観察されたectopic expressionが消失しており、内在性遺伝子の発現特性をより反映している。ホモマウスは生存性、繁殖性ともにあり。
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
Cortex-restricted disruption of NMDAR1 impairs neuronal patterns in the barrel cortex.
Iwasato T, Datwani A, Wolf A M, Nishiyama H, Taguchi Y, Tonegawa S, Knopfel T, Erzurumlu R S, Itohara S
Nature, 406, 726-731 (2000). 10963597

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA6
Emx1empty spiracles homeobox 16Emx1targeted mutation 1 (cre), Shigeyoshi Itohara
Frtyeast FRT (flippase recombination target) site6Frt
Frtyeast FRT (flippase recombination target) site6Frt
crePhage P1 Cre recombinase6cremouse Emx1 (Drosophila homeobox gene empty spiracles (Ems) mouse homolog) promoter
neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)
nlsSV40 large T antigen nuclear localization signal (NLS)6nls

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    供与核酸Simian virus 40 Large T antigen nuclear localization signal (NLS), phage P1 Cre recombinase, SV40 poly A, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Emx1 (Drosophila homeobox gene empty spiracles (Ems) mouse homolog) genomic DNA
    Research applicationCell Biology Research
    Cre/loxP system
    FLP/frt system
    Neurobiology Research
    提供条件条件を付加する。
    研究成果の公表にあたって寄託者の指定する文献を引用する。Nature, 406, 726-731 (2000).
    Depositor糸原　重美（理化学研究所脳科学総合研究センター）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month June 2006

    BRC mice in Publications

    Imayoshi I, Shimogori T, Ohtsuka T, Kageyama R.
    Hes genes and neurogenin regulate non-neural versus neural fate specification in the dorsal telencephalic midline.
    Development 135(15) 2531-41(2008) 18579678
    Kita A, Imayoshi I, Hojo M, Kitagawa M, Kokubu H, Ohsawa R, Ohtsuka T, Kageyama R, Hashimoto N.
    Hes1 and Hes5 control the progenitor pool, intermediate lobe specification, and posterior lobe formation in the pituitary development.
    Mol Endocrinol 21(6) 1458-66(2007) 17426285
    Kinameri E, Inoue T, Aruga J, Imayoshi I, Kageyama R, Shimogori T, Moore AW.
    Prdm proto-oncogene transcription factor family expression and interaction with the Notch-Hes pathway in mouse neurogenesis.
    PLoS One 3(12) e3859(2008) 19050759