Strain Information | |
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Image | |
BRC No. | RBRC00804 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | B6.129P2-S100b<tm1Ito>/ItoRbrc |
Former Common name | S100b-KO |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | C57BL/6JJcl |
Appearance | black [a/a B/B C/C] |
Strain development | Developed Hiroshi Nishiyama and Shigeyoshi Itohara, Brain Science Institute, RIKEN. E14 ES cells were used. The mutant mice were backcrossed to C57BL/6. |
Strain description | S100b gene knockout mice. Exon 2 and part of exon 3 of S100b gene were replaced with a floxed neo cassette. Homozygous mutant mice are viable and fertile. |
Colony maintenance | Sibling Mating |
References | Glial protein S100B modulates long-term neuronal synaptic plasticity. Nishiyama H, Knopfel T, Endo S, Itohara S Proc. Natl. Acad. Sci. USA, 99, 4037-4042 (2002). 11891290 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
S100b | S100 protein, beta polypeptide, neural | 10 | S100b | targeted mutation 1, Shigeyoshi Itohara | |||
loxP | phage P1 loxP | 10 | loxP | ||||
loxP | phage P1 loxP | 10 | loxP | ||||
neo | neomycin resistance gene (E. coli) | 10 | mouse phosphoglycerate kinase promoter (PGK promoter) |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neomycin, mouse calcium-binding protein S100b genomic DNA |
Research application | Cre/loxP system Neurobiology Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 99, 4037-4042 (2002). |
Depositor | Shigeyoshi Itohara (RIKEN) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Liu J, Wang H, Zhang L, Xu Y, Deng W, Zhu H, Qin C. S100B transgenic mice develop features of Parkinson's disease. Arch Med Res 42(1) 1-7(2011) 21376255 |