Strain Data Sheet

RBRC00785

Information1

Image
BRC No.RBRC00785
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(GOFMBD)2Imeg
Former Common nameOct-MBDGFP#2, TgN(GOFMBD)2Imeg
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack
Strain developmentDeveloped by Kuniya Abe, RIKEN BioResrouce Center (Institute of Molecular Embryology and Genetics) in 2001.
Strain descriptionGOFMBD transgenic mice expressing MBD (methylated DNA bifing domain) and GFP fusion protein under the control of the Oct-4 promoter (GOF18). GODMBD lines containing a proximal enhancer (PE): Line 1, Line 2, Line 4, Line 5. deltaMBD lines lacking a proximal enhancer (PE): Line 1 (RBRC00788), Line 2 (RBRC00789), Line 3 (RBRC00790).
Colony maintenanceCarrier x Carrier [or Crossing to C57BL/6JJcl]
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)UNOct 3/4 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
MBD (methylated DNA binding domain)UN

Information2

Donor DNAOct3/4 promoter, MBD(methylated DNA binding domain) cDNA, Jellyfish GFP cDNA
Research applicationCell Biology Research
Developmental Biology Research
Fluorescent Proteins/lacZ System
Genetics Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The DEPOSITOR should be a co-author in articles when the users publish their data using these mice for 2 year after provided to the RIKEN BRC.
Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice.
DepositorKuniya Abe (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
  5. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP

BRC mice in Publications

No Data