Strain Data Sheet

RBRC00771

Strain Information

Image
BRC No.RBRC00771
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(GOFGFP)11Imeg
Former Common nameOct-GFP#11, TgN(GOFGFP)11Imeg
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack
Strain developmentDeveloped by Kuniya Abe, RIKEN BioResrouce Center (Institute of Molecular Embryology and Genetics) in 2001. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs.
Strain descriptionOct-4/EGFP transgenic mice carry EGFP (enhanced green fluorescence protein) cDNA under control of an Oct4 18-kb genomic fragment containing the minimal promoter and proximal and distal enhanced. (Dev Biol 2003). The Oct-4 gene expresses in primordial germ cells and undifferentiated spermatogonia in new born mice. This strain is useful to isolate living primordial germ cells which specifically express green fluorescent protein at various stages of embryonic development.
Colony maintenanceCarrier x Carrier [or Crossing to C57BL/6JJcl]
References
Identification and characterization of stem cells in prepubertal spermatogenesis in mice.
Ohbo K, Yoshida S, Ohmura M, Ohneda O, Ogawa T, Tsuchiya H, Kuwana T, Kehler J, Abe K, Scholer H R, Suda T
Dev. Biol., 258, 209-225 (2003). 12781694

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFPGreen Fluorescent Protein (Aequorea victoria)UNOct 3/4 promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAOct 3/4 promoter, Jellyfish GFP cDNA, SV40 polyA
    Research applicationCell Biology Research
    Developmental Biology Research
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Identification and characterization of stem cells in prepubertal spermatogenesis in mice. Dev. Biol., 258, 209-225 (2003). The DEPOSITOR should be a co-author in articles when the users publish their data using these mice for 1 year after provided to the RIKEN BRC.
    Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice.
    DepositorKuniya Abe (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Li Y, Zhang Q, Yin X, Yang W, Du Y, Hou P, Ge J, Liu C, Zhang W, Zhang X, Wu Y, Li H, Liu K, Wu C, Song Z, Zhao Y, Shi Y, Deng H.
    Generation of iPSCs from mouse fibroblasts with a single gene, Oct4, and small molecules.
    Cell Res 21(1) 196-204(2011) 20956998
    Yamagata K, Ueda J, Mizutani E, Saitou M, Wakayama T.
    Survival and death of epiblast cells during embryonic stem cell derivation revealed by long-term live-cell imaging with an Oct4 reporter system.
    Dev Biol 346(1) 90-101(2010) 20659443
    Qing T, Liu H, Wei W, Ye X, Shen W, Zhang D, Song Z, Yang W, Ding M, Deng H.
    Mature oocytes derived from purified mouse fetal germ cells.
    Hum Reprod 23(1) 54-61(2008) 18000170
    Mizuno N, Kosaka M.
    Novel variants of Oct-3/4 gene expressed in mouse somatic cells.
    J Biol Chem 283(45) 30997-1004(2008) 18765667
    Yang W, Wei W, Shi C, Zhu J, Ying W, Shen Y, Ye X, Fang L, Duo S, Che J, Shen H, Ding S, Deng H.
    Pluripotin combined with leukemia inhibitory factor greatly promotes the derivation of embryonic stem cell lines from refractory strains.
    Stem Cells 27(2) 383-9(2009) 19056907