Strain Data Sheet

RBRC00762

Strain Information

Image
BRC No.RBRC00762
TypeSpontaneous Mutation
SpeciesMus musculus
Strain nameC;B6-a/a-Tyrp1<b>-Kit<W-sh>/Rbrc
Former Common nameW Sash Brown
H-2 Haplotype
ES Cell line
Background strain
Appearancewhite coat with black eyes [a/a b/b C/C sh/sh]
Strain developmentC57BL/6-Kit<W-sh> mice were deposited by Dr. Yukihiko Kitamuta, Osaka University to the RIKEN BRC. The mice were backcrossed to BALB/c for several generation, then maintained by sister-brother mating as homozygous state.
Strain descriptionThe Kit<W-sh> mutation arose spontaneously in the (C3H/HeN x 101/H)F1 at the MRC (Lyon et al., 1982). Homozygous mice are white coat with black eyes. Heterozygotes are black with a white sash. This strain is useful for study of melanogenesis and neural crest migration.
Colony maintenanceSibling mating (Homozygote x Homozygote)Homozygous mutant mice are viable and fertile.
References
The W(sh), W(57), and Ph Kit expression mutations define tissue-specific control elements located between -23 and -154 kb upstream of Kit.
Berrozpe G, Timokhina I, Yukl S, Tajima Y, Ono M, Zelenetz A D, Besmer P
Blood, 94, 2658-2666 (1999). 10515869

Mast cell-deficient W-sash c-kit mutant Kit W-sh/W-sh mice as a model for investigating mast cell biology in vivo.
Grimbaldeston M A, Chen C C, Piliponsky A M, Tsai M, Tam S Y, Galli S J
Am. J. Pathol., 167, 835-848 (2005). 16127161

c-kit Gene was not transcribed in cultured mast cells of mast cell-deficient Wsh/Wsh mice that have a normal number of erythrocytes and a normal c-kit coding region.
Tono T, Tsujimura T, Koshimizu U, Kasugai T, Adachi S, Isozaki K, Nishikawa S, Morimoto M, Nishimune Y, Nomura S, et a l
Blood, 15, 1448-1453 (1992) 1381627

W-sash affects positive and negative elements controlling c-kit expression: ectopic c-kit expression at sites of kit-ligand expression affects melanogenesis.
Duttlinger R, Manova K, Chu T Y, Gyssler C, Zelenetz A D, Bachvarova R F, Besmer P
Development, 118, 705-717 (1993). 7521281

C-kit gene is expressed by skin mast cells in embryos but not in puppies of Wsh/Wsh mice: age-dependent abolishment of c-kit gene expression.
Yamazaki M, Tsujimura T, Morii E, Isozaki K, Onoue H, Nomura S, Kitamura Y
Blood, 83, 3509-3516 (1994). 7515715

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
anonagouti2anonagouti
  • acute myeloid leukemia(DisGeNET, MedGEN)

  • cutaneous mastocytosis(MedGEN)

  • gastrointestinal stromal tumor(DisGeNET, MedGEN)
    • more 4 Diseases
  • mastocytosis(DisGeNET)

  • oculocutaneous albinism type 3(MedGEN)

  • piebaldism(DisGeNET, MedGEN)

  • testicular germ cell tumor(MedGEN)
    • Tyrp1tyrosinase-related protein 14Tyrp1brown
  • acute myeloid leukemia(DisGeNET, MedGEN)

  • cutaneous mastocytosis(MedGEN)

  • gastrointestinal stromal tumor(DisGeNET, MedGEN)
    • more 4 Diseases
  • mastocytosis(DisGeNET)

  • oculocutaneous albinism type 3(MedGEN)

  • piebaldism(DisGeNET, MedGEN)

  • testicular germ cell tumor(MedGEN)
    • KitKIT proto-oncogene receptor tyrosine kinase5Kitsash
  • acute myeloid leukemia(DisGeNET, MedGEN)

  • cutaneous mastocytosis(MedGEN)

  • gastrointestinal stromal tumor(DisGeNET, MedGEN)
    • more 4 Diseases
  • mastocytosis(DisGeNET)

  • oculocutaneous albinism type 3(MedGEN)

  • piebaldism(DisGeNET, MedGEN)

  • testicular germ cell tumor(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal coat/hair pigmentation(MP:0002075)

  • abnormal definitive hematopoiesis(MP:0002123)

  • abnormal erythrocyte cell number(MP:0001586)

  • abnormal vascular permeability(MP:0001619)

  • absent coat pigmentation(MP:0005171)
    • more 7 phenotypes
  • decreased erythrocyte cell number(MP:0002875)

  • decreased mast cell degranulation(MP:0008765)

  • decreased mast cell number(MP:0000336)

  • decreased susceptibility to type I hypersensitivity reaction(MP:0005597)

  • impaired behavioral response to xenobiotic(MP:0009747)

  • increased susceptibility to parasitic infection(MP:0005027)

  • variable body spotting(MP:0002940)
    • Detailed phenotype data

    Ordering Information

    Donor DNA
    Research applicationReproductive Biology Research
    Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
    DepositorRIKEN BRC (RIKEN BRC)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Iino S, Horiguchi S, Horiguchi K, Hashimoto T.
    Interstitial cells of Cajal in Wsh/Wsh c-kit mutant mice.
    J Smooth Muscle Res 56(0) 58-68(2020) 33132281
    Iino S, Horiguchi K, Nojyo Y.
    W(sh)/W(sh) c-Kit mutant mice possess interstitial cells of Cajal in the deep muscular plexus layer of the small intestine.
    Neurosci Lett 459(3) 123-6(2009) 19427361