Strain Data Sheet

RBRC00760

Strain Information

Image
BRC No.RBRC00760
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S-Gjc1<tm1Kni>/KniRbrc
Former Common nameB6;129S-Gja7<tm1Kni>/KniRbrc, B6;129S-Gjc1<tm1Kni>/KniRbrc, Cx45 Knockout mice
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strainC57BL/6NCrlCrlj
Appearance
Strain development卵を解凍して生まれた仔は C57BL6N Crj に6回戻し交配している。
Strain description
Colony maintenanceホモ接合体は致死のため、ヘテロ接合体で維持する必要がある。
ReferencesDevelopment, 127, 3501-3512 (2000). 10903175

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gjc1gap junction membrane channel protein alpha 711Gjc1<tm1Kni>targeted mutation 1, Kiyomasa Nishiiconnexin45(Cx45) ; Cnx45, connexin 45, Cx45, Gja-7, Gja7

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)11

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)11nls

Ordering Information

供与核酸mouse connexin45(Cx45) genomic DNA, Simian virus 40 Large T antigen nuclear localization signal (NLS), mouse phosphoglycerate kinase promoter (PGK promoter), Phage P1 loxP, E. coli neo, E. coli lacZ
Research application
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Development, 127, 3501-3512 (2000).
Depositor西井　清雅（防衛医科大学校）
Strain Status凍結胚のアイコン凍結胚
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data