Strain Data Sheet



BRC No.RBRC00754
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Cd40<tm1Kik> Tg(Igh-CD40)1Kik/Rbrc
Former Common nameCD40 transgenic mice
H-2 Haplotype
ES Cell line
Background strain
Strain developmentDeveloped by Hitoshi Kikutani, Research Institute for Microbial Diseases, Osaka University in 2002.
Strain descriptionTransgenic mice expressing wild-type human CD40 gene in a mouse CD40 deficient background. CD40/Tnfrsf5 (tumor necrosis factor receptor superfamily, member 5) is essential for T cell-dependent immunoglobulin class switching and germinal center formation.
Colony maintenance
ReferencesInt. Immunol., 14, 319-329 (2002). 11867568
Nature, 316, 67-69 (1985). 2409451

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cd40CD40 antigen2Cd40<tm1Kik>targeted mutation 1, Hitoshi KikutaniBp50, Cd40, p50, Tnfrsf5

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)2neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
CD40CD40 antigen (human)UNCD40CD40, p50, TNFRSF5mouse E m, IgH promoter, rabbit beta-globin poly A


Donor DNAmouse Em, IgH promoter, human CD40 cDNA, rabbit beta-globin poly A, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse CD40 genomic DNA
Research applicationImmunology and Inflammation Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Inter. Immunol., 14, 319-329 (2002).
DepositorHitoshi Kikutani (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data