Strain Data Sheet

RBRC00752

Strain Information

Image
BRC No.RBRC00752
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Cd40<tm1Kik>/Rbrc
Former Common nameCD40 Knockout mice, B6.129P2-CD40<tm1Kik>/Rbrc, B6.129P2-Tnfrsf5<tm1Kik>/Rbrc
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain development大阪大学細胞工学センター・菊谷　仁先生（1994）。
Strain descriptionCD40ノックアウトマウス。エクソン3をneoカセットで置換。
Colony maintenance
References
The immune responses in CD40-deficient mice: impaired immunoglobulin class switching and germinal center formation.
T Kawabe, T Naka, K Yoshida, T Tanaka, H Fujiwara, S Suematsu, N Yoshida, T Kishimoto, H Kikutani
Immunity, 1, 167-178 (1994). 7534202

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cd40
MGI:88336		CD40 antigen2Cd40<tm1Kik>
MGI:1857457		targeted mutation 1, Hitoshi Kikutani
  • hyper-IgM syndrome type 3(MedGEN)
    • neo neomycin resistance gene (E. coli)2 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal B cell activation (MP:0008217)

  • abnormal class switch recombination (MP:0004816)

  • abnormal dendritic cell physiology (MP:0002376)

  • abnormal humoral immune response (MP:0001800)

  • absent germinal center B cells (MP:0008179)
    • more 23 phenotypes
  • absent spleen germinal center (MP:0008474)

  • decreased B cell number (MP:0005017)

  • decreased IgA level (MP:0001807)

  • decreased IgE level (MP:0002492)

  • decreased IgG1 level (MP:0008495)

  • decreased IgG2a level (MP:0008496)

  • decreased IgG2b level (MP:0008497)

  • decreased IgG3 level (MP:0008498)

  • decreased bone mineral density (MP:0000063)

  • decreased cerebral infarct size (MP:0006058)

  • decreased compact bone mass (MP:0010962)

  • decreased immunoglobulin level (MP:0002460)

  • decreased neutrophil cell number (MP:0000222)

  • decreased platelet aggregation (MP:0009549)

  • decreased susceptibility to type II hypersensitivity reaction (MP:0005612)

  • decreased trabecular bone mass (MP:0010873)

  • glomerulonephritis (MP:0002743)

  • hematopoietic system phenotype (MP:0005397)

  • immune system phenotype (MP:0005387)

  • increased anti-double stranded DNA antibody level (MP:0004762)

  • increased marginal zone B cell number (MP:0008181)

  • increased susceptibility to bacterial infection (MP:0002412)

  • increased urine protein level (MP:0002962)
    • Detailed phenotype data

    Ordering Information

    供与核酸herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Tnfrsf5 genomic DNA
    Research applicationImmunology and Inflammation Research
    提供条件研究成果の公表にあたって寄託者の指定する文献を引用する。Immunity, 1, 167-178 (1994).
    Depositor菊谷　仁（大阪大学）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications


    Synergistic activation of STING and CD40 in the tumor microenvironment enhances CD8+ T-cell-dependent antitumor immunity
    Biochem Biophys Res Commun (2025)