Strain Data Sheet


Strain Information

BRC No.RBRC00752
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Cd40<tm1Kik>/Rbrc
Former Common nameCD40 Knockout mice, B6.129P2-CD40<tm1Kik>/Rbrc, B6.129P2-Tnfrsf5<tm1Kik>/Rbrc
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Strain developmentDeveloped by Hitoshi Kikutani, Institute for Molecular and Cellular Biology, Osaka University in 1994.
Strain descriptionCD40 knockout mice. Exon 3 of the CD40 was replaced with a neo cassette. CD40/Tnfrsf5 (tumor necrosis factor receptor superfamily, member 5) is essential for T cell-dependent immunoglobulin class switching and germinal center formation. Homozygous mutant mice for CD40 targeted mutation exhibit impaired immunoglobulin class switching, and the germinal center formation was defective. Homozygous mice are viable and fertile.
Colony maintenance
ReferencesImmunity, 1, 167-178 (1994). 7534202

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cd40CD40 antigen2Cd40<tm1Kik>targeted mutation 1, Hitoshi KikutaniBp50, Cd40, p50, Tnfrsf5

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)2neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Ordering Information

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Tnfrsf5 genomic DNA
Research applicationImmunology and Inflammation Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Immunity, 1, 167-178 (1994).
DepositorHitoshi Kikutani (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data