Strain Data Sheet

RBRC00752

Strain Information

Image
BRC No.RBRC00752
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Cd40<tm1Kik>/Rbrc
Former Common nameCD40 Knockout mice, B6.129P2-CD40<tm1Kik>/Rbrc, B6.129P2-Tnfrsf5<tm1Kik>/Rbrc
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Hitoshi Kikutani, Institute for Molecular and Cellular Biology, Osaka University in 1994.
Strain descriptionCD40 knockout mice. Exon 3 of the CD40 was replaced with a neo cassette. CD40/Tnfrsf5 (tumor necrosis factor receptor superfamily, member 5) is essential for T cell-dependent immunoglobulin class switching and germinal center formation. Homozygous mutant mice for CD40 targeted mutation exhibit impaired immunoglobulin class switching, and the germinal center formation was defective. Homozygous mice are viable and fertile.
Colony maintenance
References
The immune responses in CD40-deficient mice: impaired immunoglobulin class switching and germinal center formation.
Kawabe T, Naka T, Yoshida K, Tanaka T, Fujiwara H, Suematsu S, Yoshida N, Kishimoto T, Kikutani H
Immunity, 1, 167-178 (1994). 7534202

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cd40CD40 antigen2Cd40targeted mutation 1, Hitoshi Kikutani
  • hyper-IgM syndrome type 3(MedGEN)
    • neoneomycin resistance gene (E. coli)2herpes simplex virus thymidine kinase promoter (HSV tk promoter)
  • hyper-IgM syndrome type 3(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal B cell activation(MP:0008217)

  • abnormal class switch recombination(MP:0004816)

  • abnormal dendritic cell physiology(MP:0002376)

  • abnormal humoral immune response(MP:0001800)

  • absent germinal center B cells(MP:0008179)
    • more 23 phenotypes
  • absent spleen germinal center(MP:0008474)

  • decreased B cell number(MP:0005017)

  • decreased IgA level(MP:0001807)

  • decreased IgE level(MP:0002492)

  • decreased IgG1 level(MP:0008495)

  • decreased IgG2a level(MP:0008496)

  • decreased IgG2b level(MP:0008497)

  • decreased IgG3 level(MP:0008498)

  • decreased bone mineral density(MP:0000063)

  • decreased cerebral infarct size(MP:0006058)

  • decreased compact bone mass(MP:0010962)

  • decreased immunoglobulin level(MP:0002460)

  • decreased neutrophil cell number(MP:0000222)

  • decreased platelet aggregation(MP:0009549)

  • decreased susceptibility to type II hypersensitivity reaction(MP:0005612)

  • decreased trabecular bone mass(MP:0010873)

  • glomerulonephritis(MP:0002743)

  • hematopoietic system phenotype(MP:0005397)

  • immune system phenotype(MP:0005387)

  • increased anti-double stranded DNA antibody level(MP:0004762)

  • increased marginal zone B cell number(MP:0008181)

  • increased susceptibility to bacterial infection(MP:0002412)

  • increased urine protein level(MP:0002962)
    • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Tnfrsf5 genomic DNA
    Research applicationImmunology and Inflammation Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Immunity, 1, 167-178 (1994).
    DepositorHitoshi Kikutani (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data