Strain Data Sheet

RBRC00702

Strain Information

Image
BRC No.RBRC00702
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S4-Zic1<tm1Jaru>/JaruRbrc
Former Common nameC57BL/6-TgH(Zic1)
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strainC57BL/6JJcl
Appearanceblack
Strain developmentDeveloped by Aruga J (Aruga J et al. J Neurosci 18, 284-293, 1998).The mice were frozen-stored at 2-cell stage after in vitro fertilization between a heterozygous male and C57BL/6J females. Live mice were recovered and born on April 28, 2003.Deposited to RIKEN BioResource Center, RIKEN Tsukuba Institute in 2002.
Strain descriptionZic genes encode zinc finger proteins. These genes are homologs of the Drosophila pair-rule gene odd-paired. This strain was developed to clarify the function of Zic1 gene. Homozygous Zic1 deficient mice show remarkable ataxia during postnatal development, and die within 1 month. Their cerebella are hypoplastic and missing a lobule in the anterior lobe. A bromodeoxyuridine labeling study indicated a reduction both in the proliferating cell fraction in the external germinal layer (EGL), from embryonic day14, and in forward movement of the EGL (Aruga J et al. J Neurosci 18, 284-293, 1998).
Colony maintenanceMice were maintained by successive backcrossing heterozygotes to C57BL/6 mice.
References
Mouse Zic1 is involved in cerebellar development.
Aruga J, Minowa O, Yaginuma H, Kuno J, Nagai T, Noda T, Mikoshiba K
J. Neurosci., 18, 284-293 (1998). 9412507

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Zic1
MGI:106683		zinc finger protein of the cerebellum 19Zic1<tm1Jaru>
MGI:2156822		targeted mutation 1, Jun Aruga
  • craniosynostosis 6(MedGEN)

  • structural brain anomalies with impaired intellectual development and craniosynostosis(MedGEN)
    • neo neomycin resistance gene (E. coli)9 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal cerebellar foliation (MP:0000857)

  • abnormal cerebellum development (MP:0000854)

  • abnormal cerebellum vermis morphology (MP:0000864)

  • abnormal gait (MP:0001406)

  • abnormal suckling behavior (MP:0001436)
    • more 12 phenotypes
  • ataxia (MP:0001393)

  • cerebellum hypoplasia (MP:0000851)

  • decreased locomotor activity (MP:0001402)

  • hypotonia (MP:0004144)

  • impaired limb coordination (MP:0001524)

  • impaired righting response (MP:0001523)

  • kyphosis (MP:0000160)

  • neonatal lethality, incomplete penetrance (MP:0011088)

  • postnatal growth retardation (MP:0001732)

  • postnatal lethality, complete penetrance (MP:0011085)

  • premature death (MP:0002083)

  • reduced cerebellar foliation (MP:0009719)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse zinc finger protein of the cerebellum 1(nervous system specific transcription factor) Zic1 genomic DNA, mouse phosphoglycerate kinase promoter, E. coli neo
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 18, 284-293 (1998). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorJun Aruga (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP

    BRC mice in Publications

    Grinberg I, Northrup H, Ardinger H, Prasad C, Dobyns WB, Millen KJ.
    Heterozygous deletion of the linked genes ZIC1 and ZIC4 is involved in Dandy-Walker malformation.
    Nat Genet 36(10) 1053-5(2004) 15338008