Strain Data Sheet

RBRC00673

Strain Information

Image
BRC No.RBRC00673
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Stam<tm2Sug> Stam2<tm1Sug>/SugRbrc
Former Common nameB6.Cg-Stam<tm2Sug> Stam2<tm1Sug>, STAM1<flox>mice
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strainC57BL/6NCrSlc
Appearanceblack
Strain development東北大学大学院医学系研究科・菅村和夫先生（2001）。C57BL/6N背景（N7以上）。
Strain descriptionSTAM1 floxed、STAM2 KO2重変異マウス。T細胞特異的 (Lck-Cre Tg) にSTAM1flox/floxとSTAM2-/-を2重欠損させるとT細胞分化異常を呈し、成熟T細胞が欠損する。
Colony maintenanceStam: Homozygote x Homozygote [or Crossing to C57BL/6NCrSlc]; Stam2: Homozygote x Homozygote [or Crossing to C57BL/6NCrSlc]
References
Signal-transducing adaptor molecules STAM1 and STAM2 are required for T-cell development and survival.
Mitsuhiro Yamada, Naoto Ishii, Hironobu Asao, Kazuko Murata, Chieko Kanazawa, Hidetada Sasaki, Kazuo Sugamura
Mol Cell Biol., 22(24): 8648-8658 (2002). 12446783

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Stam
MGI:1329014		signal transducing adaptor molecule (SH3 domain and ITAM motif) 12Stam<tm2Sug>
MGI:2446572		targeted mutation 2, Kazuo Sugamura
Stam2
MGI:1929100		signal transducing adaptor molecule (SH3 domain and ITAM motif) 22Stam2<tm1Sug>
MGI:2446571		targeted mutation 1, Kazuo Sugamura
loxP phage P1 loxP2loxP
loxP phage P1 loxP2loxP
loxP phage P1 loxP2loxP
neo neomycin resistance gene (E. coli)2 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal T cell differentiation (MP:0002145)

  • decreased T cell number (MP:0005018)

  • decreased T cell proliferation (MP:0005095)

  • embryonic lethality during organogenesis, complete penetrance (MP:0011098)
    • Detailed phenotype data

    Ordering Information

    供与核酸phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse STAM1 and STAM2 genomic DNA
    Research applicationCre/loxP system
    Neurobiology Research
    提供条件利用者は事前に寄託者に利用の報告をする。公表を前提とした学術研究に限る。利用者が当該リソースを使用した研究成果に基づく特許等の申請を行う場 合は、寄託者と別途協議を行う。
    Depositor菅村　和夫（地方独立行政法人宮城県立病院機構）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data