Strain Data Sheet

RBRC00673

Strain Information

Image
BRC No.RBRC00673
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Stam<tm2Sug> Stam2<tm1Sug>/SugRbrc
Former Common nameB6.Cg-Stam<tm2Sug> Stam2<tm1Sug>, STAM1<flox>mice
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strainC57BL/6NCrSlc
Appearanceblack
Strain developmentDeveloped by Kazuo Sugamura, Tohoku University Graduate School of Medicine. The mutant mice were backcrossed to C57BL/6N over 7 times.
Strain descriptionSTAM1 floxed, STAM2 KO double mutant mice.
Colony maintenanceStam: Homozygote x Homozygote [or Crossing to C57BL/6NCrSlc]; Stam2: Homozygote x Homozygote [or Crossing to C57BL/6NCrSlc]
References
Signal-transducing adaptor molecules STAM1 and STAM2 are required for T-cell development and survival.
Yamada M, Ishii N, Asao H, Murata K, Kanazawa C, Sasaki H, Sugamura K
Mol Cell Biol., 22(24): 8648-8658 (2002). 12446783

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Stamsignal transducing adaptor molecule (SH3 domain and ITAM motif) 12Stamtargeted mutation 2, Kazuo Sugamura
Stam2signal transducing adaptor molecule (SH3 domain and ITAM motif) 22Stam2targeted mutation 1, Kazuo Sugamura
loxPphage P1 loxP2loxP
loxPphage P1 loxP2loxP
loxPphage P1 loxP2loxP
neoneomycin resistance gene (E. coli)2mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal T cell differentiation(MP:0002145)

  • decreased T cell number(MP:0005018)

  • decreased T cell proliferation(MP:0005095)

  • embryonic lethality during organogenesis, complete penetrance(MP:0011098)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse STAM1 and STAM2 genomic DNA
    Research applicationCre/loxP system
    Neurobiology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall prior inform the DEPOSITOR of the purpose of the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorKazuo Sugamura (Miyagi Prefectural Hospital Organization)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data