Strain Data Sheet

RBRC00672

Strain Information

Image
BRC No.RBRC00672
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.Cg-Stam<tm1Sug> Stam2<tm1Sug>/SugRbrc
Former Common nameC.Cg-Stam<tm1Sug> Stam2<tm1Sug>, STAM1+/-, STAM2+/-
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strainBALB/c
Appearancealbino
Strain developmentDeveloped by Kazuo Sugamura, Tohoku University Graduate School of Medicine in 1999. The mutant mice were backcrossed to BALB/c over 6 times.
Strain descriptionSTAM1, STAM2 double knockout mice. STAM1 homozygous mutant mice show growth defect with aging, and neurological abnormality (reduction of pyramidal cells in CA3 of the hippocampus). STAM2 homozygous mutant mice are indistinguishable from controls. Double homozygous mutant mice are embryonic lethal.
Colony maintenanceStam: Heterozygote x Wild-type [or Crossing to BALB/cCrSlc]; Stam2: Homozygote x Homozygote [or Crossing to BALB/cCrSlc]
References
Signal-transducing adaptor molecules STAM1 and STAM2 are required for T-cell development and survival.
Yamada M, Ishii N, Asao H, Murata K, Kanazawa C, Sasaki H, Sugamura K
Mol. Cell. Biol., 22, 8648-8658 (2002). 12446783

Loss of hippocampal CA3 pyramidal neurons in mice lacking STAM1.
Yamada M, Takeshita T, Miura S, Murata K, Kimura Y, Ishii N, Nose M, Sakagami H, Kondo H, Tashiro F, Miyazaki J I, Sasaki H, Sugamura K
Mol. Cell. Biol., 21, 3807-3819 (2001). 11340172

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Stamsignal transducing adaptor molecule (SH3 domain and ITAM motif) 12Stamtargeted mutation 1, Kazuo Sugamura
Stam2signal transducing adaptor molecule (SH3 domain and ITAM motif) 22Stam2targeted mutation 1, Kazuo Sugamura
loxPphage P1 loxP2loxP
loxPphage P1 loxP2loxP
neoneomycin resistance gene (E. coli)2mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • decreased T cell number(MP:0005018)

  • decreased T cell proliferation(MP:0005095)

  • decreased hippocampus pyramidal cell number(MP:0009971)

  • embryonic lethality during organogenesis, complete penetrance(MP:0011098)

  • embryonic lethality(MP:0008762)
    • more 7 phenotypes
  • gliosis(MP:0002183)

  • increased susceptibility to pharmacologically induced seizures(MP:0002906)

  • limb grasping(MP:0001513)

  • male infertility(MP:0001925)

  • postnatal growth retardation(MP:0001732)

  • premature death(MP:0002083)

  • priapism(MP:0003415)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse STAM1 and STAM2 genomic DNA
    Research applicationCre/loxP system
    Neurobiology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall prior inform the DEPOSITOR of the purpose of the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorKazuo Sugamura (Miyagi Prefectural Hospital Organization)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data