Strain Data Sheet

RBRC00671

Strain Information

Image
BRC No.RBRC00671
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Amd1<tm1Kiga>/Rbrc
Former Common namemouse S-adenosylmethionine decarboxylase gene Knockout mice
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strainC57BL/6
Appearanceblack
Strain developmentES細胞は129系。バッククロスに用いたのはC57BL/6
Strain descriptionヘテロ変異は、NO Phenotype.ホモ変異は、E3.5以降での胚性致死。
Colony maintenanceヘテロ変異マウスとWild Typeマウスの交配により、系統維持。
References
Essential role of S-adenosylmethionine decarboxylase in mouse embryonic development.
Nishimura K, Nakatsu F, Kashiwagi K, Ohno H, Saito T, Igarashi K
Genes Cells, 7, 41-47 (2002). 11856372

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Amd1
MGI:88004		S-adenosylmethionine decarboxylase 110Amd1<tm1Kiga>
MGI:3586494		targeted mutation 1, Kazuei Igarashi
GFP Green Fluorescent Protein (Aequorea victoria)10
loxP phage P1 loxP10loxP
loxP phage P1 loxP10loxP
neo neomycin resistance gene (E. coli)10

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • embryonic growth arrest(MP:0001730)

  • embryonic lethality between implantation and somite formation, complete penetrance(MP:0011096)
    • Detailed phenotype data

    Ordering Information

    供与核酸mouse S-adenosylmethionine decarboxylase genomic DNA, Jellyfish GFP cDNA, E. coli neo, Phage P1 loxP, herpes simplex virus thymidine kinase promoter (HSV tk promoter)
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    提供条件利用者は事前に寄託者の提供承諾書を得る。分譲に当たっては寄託後、寄託者との共同研究とする。
    利用者が当該リソースを使用した研究成果に基づく特許等の申請を行う場合は、寄託者と別途協議を行う。
    Depositor五十嵐　一衛（千葉大学）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data