Strain Data Sheet

RBRC00671

Strain Information

Image
BRC No.RBRC00671
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Amd1<tm1Kiga>/Rbrc
Former Common namemouse S-adenosylmethionine decarboxylase gene Knockout mice
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strainC57BL/6
Appearanceblack
Strain developmentES細胞は129系。バッククロスに用いたのはC57BL/6
Strain descriptionヘテロ変異は、NO Phenotype.ホモ変異は、E3.5以降での胚性致死。
Colony maintenanceヘテロ変異マウスとWild Typeマウスの交配により、系統維持。
References
Essential role of S-adenosylmethionine decarboxylase in mouse embryonic development.
Nishimura K, Nakatsu F, Kashiwagi K, Ohno H, Saito T, Igarashi K
Genes Cells, 7, 41-47 (2002). 11856372

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Amd1S-adenosylmethionine decarboxylase 110Amd1targeted mutation 1, Kazuei Igarashi
GFPGreen Fluorescent Protein (Aequorea victoria)10
loxPphage P1 loxP10loxP
loxPphage P1 loxP10loxP
neoneomycin resistance gene (E. coli)10

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • embryonic growth arrest(MP:0001730)

  • embryonic lethality between implantation and somite formation, complete penetrance(MP:0011096)
    • Detailed phenotype data

    Ordering Information

    供与核酸mouse S-adenosylmethionine decarboxylase genomic DNA, Jellyfish GFP cDNA, E. coli neo, Phage P1 loxP, herpes simplex virus thymidine kinase promoter (HSV tk promoter)
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    提供条件条件を付加する。利用者は事前に寄託者の提供承諾書を得る。
    分譲に当たっては寄託後、寄託者との共同研究とする。
    利用者が当該リソースを使用した研究成果に基づく特許等の申請を行う場合は、寄託者と別途協議を行う。
    Depositor五十嵐　一衛（千葉大学）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data