Strain Data Sheet

RBRC00522

Strain Information

Image
BRC No.RBRC00522
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;129-Rag2<tm1Fwa> Il2rb<tm1Mak>/Rbrc
Former Common nameC57BL/6-Rag2<tm1Fwa>-IL2rb<tm1Och>
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack
Strain development1998 by Koyasu at Keio Univ. School of Med.Original :Rag2tm1Fwa :1992 by F. W. Alt at Columbia Univ., N.Y. USAIL2rbtm1Och:1995 by T. W. Mak at Ontario Cancer Institute, Toronto, Canada
Strain descriptionThey lack T, B and NK cells. Furthermore, Macrophages and DCs have some functional defects.
Colony maintenanceNothing special. Breed well.
ReferencesScience, 268, 1472-1476 (1995). 7770771
Nat. Immunol., 2, 1138-1143 (2001). 11702064
J. Immunol., 157, 4379-4389 (1996). 8906813
Eur. J. Immunol., 31, 3147-3152 (2001). 11745331
Cell, 68, 855-867 (1992). 1547487

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Il2rbinterleukin 2 receptor, beta chain15Il2rb<tm1Mak>targeted mutation 1, Tak W MakCD122, IL-2 receptor beta chain, Il-2Rbeta, p70

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)15neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Rag2recombination activating gene 22Rag2<tm1Fwa>targeted mutation 1, Frederick W AltRag-2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)2neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Ordering Information

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E.coli neo, herpes simplex virus thymidine kinase polyA additional signal, mouse Rag2 genomic DNA, mouse IL2rb genomic DNA
Research applicationCell Biology Research
Immunology and Inflammation Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER (Tak W. Mak, Division of Stem Cell and Developmental Biology, Advanced Medical Discovery Institute/Ontario Cancer Institute E-mail: tmak@uhnresearch.ca).
DepositorShigeo Koyasu (Keio University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data