Strain Information | |
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Image | |
BRC No. | RBRC00522 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | B6;129-Rag2<tm1Fwa> Il2rb<tm1Mak>/Rbrc |
Former Common name | C57BL/6-Rag2<tm1Fwa>-IL2rb<tm1Och> |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | black |
Strain development | 1998 by Koyasu at Keio Univ. School of Med.Original :Rag2tm1Fwa :1992 by F. W. Alt at Columbia Univ., N.Y. USAIL2rbtm1Och:1995 by T. W. Mak at Ontario Cancer Institute, Toronto, Canada |
Strain description | They lack T, B and NK cells. Furthermore, Macrophages and DCs have some functional defects. |
Colony maintenance | Nothing special. Breed well. |
References | Deregulated T cell activation and autoimmunity in mice lacking interleukin-2 receptor beta. Suzuki H, Kundig T M, Furlonger C, Wakeham A, Timms E, Matsuyama T, Schmits R, Simard J J, Ohashi P S, Griesser H, et a l Science, 268, 1472-1476 (1995). 7770771Critical role of IL-15-IL-15R for antigen-presenting cell functions in the innate immune response. Ohteki T, Suzue K, Maki C, Ota T, Koyasu S Nat. Immunol., 2, 1138-1143 (2001). 11702064Development of CD4-CD8- alpha beta TCR+NK1.1+ T lymphocytes: thymic selection by self antigen. Schulz R J, Parkes A, Mizoguchi E, Bhan A K, Koyasu S J. Immunol., 157, 4379-4389 (1996). 8906813Critical role of NK but not NKT cells in acute rejection of parental bone marrow cells in F1 hybrid mice. Suzue K, Reinherz E L, Koyasu S Eur. J. Immunol., 31, 3147-3152 (2001). 11745331RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement. Shinkai Y, Rathbun G, Lam K P, Oltz E M, Stewart V, Mendelsohn M, Charron J, Datta M, Young F, Stall A M, et a l Cell, 68 (5) 855-67 (1992). 1547487 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Il2rb | interleukin 2 receptor, beta chain | 15 | Il2rb | targeted mutation 1, Tak W Mak | |||
neo | neomycin resistance gene (E. coli) | 15 | herpes simplex virus thymidine kinase promoter (HSV tk promoter) | ||||
Rag2 | recombination activating gene 2 | 2 | Rag2 | targeted mutation 1, Frederick W Alt | |||
neo | neomycin resistance gene (E. coli) | 2 | herpes simplex virus thymidine kinase promoter (HSV tk promoter) |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | more 82 phenotypes |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | herpes simplex virus thymidine kinase promoter (HSV tk promoter), E.coli neo, herpes simplex virus thymidine kinase polyA additional signal, mouse Rag2 genomic DNA, mouse IL2rb genomic DNA |
Research application | Cell Biology Research Immunology and Inflammation Research |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER (Tak W. Mak, Division of Stem Cell and Developmental Biology, Advanced Medical Discovery Institute/Ontario Cancer Institute E-mail: tmak@uhnresearch.ca). |
Depositor | Shigeo Koyasu (Keio University) |
Strain Status | Frozen embryos |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |