Strain Data Sheet

RBRC00468

Strain Information

Image
BRC No.RBRC00468
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(ACTB-CCL2)1Onoe/Rbrc
Former Common namehuman MCP-1 Tgm Line 1
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack
Strain development1994年、岩渕和也先生がmicroinjectionにより北海道大学免疫科学研究所（附属免疫動物実験施設）にて確立した。
Strain descriptionヒトMCP-1蛋白を血中に高濃度 (10〜17 ng/ml)に発現し、様々な臓器において発現が認められる高濃度に発現することでマクロファージの貧食機能は抑制される。接触過敏反応はTgmで増強する。
Colony maintenanceSPFで飼育。繁殖効率は良い。conventionalではwild type に比して、このTgmと同様に全身で発現するタイプは、感染のため寿命が短いという報告がある。（J. Immunol., 155, 4838, 1995).
References
A human beta-actin expression vector system directs high-level accumulation of antisense transcripts.
P Gunning, J Leavitt, G Muscat, S Y Ng, L Kedes
Proc. Natl. Acad. Sci. USA, 84, 4831-4835 (1987). 2440031

Properties of pro-inflammatory cell type-specific leukocyte chemotactic cytokines, interleukin 8 (IL-8) and monocyte chemotactic and activating factor (MCAF).
N Mukaida, A Harada, K Yasumoto, K Matsushima
Microbiol. Immunol., 36, 773-789 (1992). 1474930

High level monocyte chemoattractant protein-1 expression in transgenic mice increases their susceptibility to intracellular pathogens.
B J Rutledge, H Rayburn, R Rosenberg, R J North, R P Gladue, C L Corless, B J Rollins
J. Immunol., 155, 4838- 4843 (1995). 7594486

Delayed clearance of zymosan-induced granuloma and depressed phagocytosis of macrophages with concomitant up-regulated kinase activities of Src-family in a human monocyte chemoattractant protein-1 transgenic mouse.
M Ato, K Iwabuchi, N Matsuki, N Mukaida, C Iwabuchi, A Takahashi, T Takayanagi, E A Dondog, S Hatakeyama, H Ishikura, M Kato, I Negishi, H Nishihori, K Watano, K Ogasawara, K Matsushima, K Onoé
Immunobiol., 201, 432-449 (2000). 10776798

Enhanced contact hypersensitivity in human monocyte chemoattractant protein-1 transgenic mouse.
N Mizumoto, K Iwabichi, H Nakamura, M Ato, A Shibaki, T Kawashima, H Kobayashi, C Iwabuchi, A Ohkawara, K Onoé
Immunobiol., 204, 477-493 (2001). 11776402

Augmented expression of tumour necrosis factor-alpha induced by lipopolysaccharide in spleen of human monocyte chemoattractant protein-1 transgenic mouse enhances the lipopolysaccharide sensitivity of the marginal zone macrophages.
Manabu Ato, Kazuya Iwabuchi, Shigeki Shimada, Naofumi Mukaida, Kazunori Onoé
Immunology, 106, 554-563 (2002). 12153519

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
CCL2 chemokine (C-C motif) ligand 2 (human)UNCCL2 human beta-actin promoter

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal macrophage physiology (MP:0002451)

  • decreased survivor rate (MP:0008770)
    • Detailed phenotype data

    Ordering Information

    供与核酸human monocyte chemotactic and activatingfactor (MCP-1) cDNA, human beta actin promoter, 5'UT, IVS, SV40 poly A
    Research application
    提供条件条件を付加しない。
    Depositor岩渕　和也（北海道大学）
    Strain Status凍結胚のアイコン凍結胚
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data