Strain Data Sheet

RBRC00462

Strain Information

Image
BRC No.RBRC00462
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Mak<tm1Yshk>/YshkRbrc
Former Common nameMAK Knockout mice B6
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6NJcl
Appearanceblack [a/a B/B C/C]
Strain development1998年、日本ロシュ(株)研究所で作製。日本クレアのC57BL/6N-Jclとbackクロスを9回行ったものを理研BRCに導入。
Strain descriptionホモMAK KO オスはfertileだが、個体によっては精子の運動性が極端に悪いものがある。
Colony maintenanceヘテロマウスはAホモマウス　メスはA、オスはA(個体によってはB）
References
A testicular germ cell-associated serine-threonine kinase, MAK, is dispensable for sperm formation.
Shinkai Y, Satoh H, Takeda N, Fukuda M, Chiba E, Kato T, Kuramochi T, Araki Y
Mol. Cell. Biol., 22, 3276-3280 (2002). 11971961

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Mak
MGI:96913		male germ cell-associated kinase13Mak<tm1Yshk>
MGI:2179972		targeted mutation 1, Yoichi Shinkai
  • retinitis pigmentosa 62(MedGEN)
    • neo neomycin resistance gene (E. coli)13 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal sperm motility (MP:0002674)

  • asthenozoospermia (MP:0002675)

  • decreased litter size (MP:0001935)
    • Detailed phenotype data

    Ordering Information

    供与核酸mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse male germ cell-associated kinase (MAK) genomic DNA
    Research applicationCell Biology Research
    提供条件研究成果の公表にあたって寄託者の指定する文献を引用する。研究成果の公表にあたって謝辞の表明を必要とする。
    DepositorFrances Ford（日本ロシュ(株)研究所）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Omori Y, Chaya T, Katoh K, Kajimura N, Sato S, Muraoka K, Ueno S, Koyasu T, Kondo M, Furukawa T.
    Negative regulation of ciliary length by ciliary male germ cell-associated kinase (Mak) is required for retinal photoreceptor survival.
    Proc Natl Acad Sci U S A 107(52) 22671-6(2010) 21148103