Strain Data Sheet

RBRC00462

Strain Information

Image
BRC No.RBRC00462
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Mak<tm1Yshk>/YshkRbrc
Former Common nameMAK Knockout mice B6
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6NJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Shinkai at Kyoto University. A neo cassette was transferred into TT2 ES cells to replace exons 5-8 in the Mak gene. The mutant mice were backcrossed to C57BL/6NJcl for 9 times.
Strain descriptionB6.Cg-Mak<tm1Yshk>. Mak, male germ cell-associated kinase gene expresses specifically in testicular germ cells. Mak deficient mice show no gross abnormalities, and most of the mice were fertile. However, the litter size derived homozygous mutant males and their sperm motility in vitro were mildly reduced compared to those of normal mice. This strain is useful for elucidation of the function of Mak gene in spermatogenesis.
Colony maintenanceSibling mating.Homozygous male are fertile, but exhibit slight reduction in litter size and sperm motility in vitro.
References
A testicular germ cell-associated serine-threonine kinase, MAK, is dispensable for sperm formation.
Shinkai Y, Satoh H, Takeda N, Fukuda M, Chiba E, Kato T, Kuramochi T, Araki Y
Mol. Cell. Biol., 22, 3276-3280 (2002). 11971961

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Makmale germ cell-associated kinase13Maktargeted mutation 1, Yoichi Shinkai
  • retinitis pigmentosa 62(MedGEN)
    • neoneomycin resistance gene (E. coli)13mouse phosphoglycerate kinase promoter (PGK promoter)
  • retinitis pigmentosa 62(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal sperm motility(MP:0002674)

  • asthenozoospermia(MP:0002675)

  • decreased litter size(MP:0001935)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse male germ cell-associated kinase (MAK) genomic DNA
    Research applicationCell Biology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorFrances Ford (Kamakura Research LaboratoriesRoche)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Omori Y, Chaya T, Katoh K, Kajimura N, Sato S, Muraoka K, Ueno S, Koyasu T, Kondo M, Furukawa T.
    Negative regulation of ciliary length by ciliary male germ cell-associated kinase (Mak) is required for retinal photoreceptor survival.
    Proc Natl Acad Sci U S A 107(52) 22671-6(2010) 21148103