Strain Data Sheet

RBRC00458

Strain Information

Image
BRC No.RBRC00458
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Cdkn1b<tm1Kin>
Former Common nameB6.129P2-Cdkn1b<tm1Kin>; p27 Knockout mice
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearance
Strain developmentWashington University School of Medicine・中山敬一先生(1996)。E14 ES細胞を用いて作出。C57BL/6へ戻し交配された。
Strain descriptionp27 (Cdkn1b) 遺伝子のノックアウトマウス。エクソン1と2を含む2.5 kb領域をPGK-neoカセットで置換。
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cdkn1bcyclin-dependent kinase inhibitor 1B6Cdkn1b<tm1Kin>targeted mutation 1, Kei-ichi NakayamaKip1, p27, p27Kip1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

供与核酸mouse phosphoglycerate kinase promoter (PGK promoter), Neomycin resistance gene (neomycin phosphotransferase II (nptII) gene of the transposon Tn5 in Escherichia coli K12), mouse PGK polyA, mouse Cdkn1b genomic DNA
Research application
提供条件条件を付加する。利用者は事前に寄託者の提供承諾書を得る。
共同研究
Depositor中山 敬一(国立大学法人九州大学)
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data