Strain Data Sheet

RBRC00458

Strain Information

Image
BRC No.RBRC00458
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Cdkn1b<tm1Kin>
Former Common nameB6.129P2-Cdkn1b<tm1Kin>; p27 Knockout mice
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearance
Strain developmentWashington University School of Medicine・中山敬一先生（1996）。E14 ES細胞を用いて作出。C57BL/6へ戻し交配された。
Strain descriptionp27 (Cdkn1b) 遺伝子のノックアウトマウス。エクソン1と2を含む2.5 kb領域をPGK-neoカセットで置換。
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Mice lacking p27(Kip1) display increased body size, multiple organ hyperplasia, retinal dysplasia, and pituitary tumors.
K Nakayama, N Ishida, M Shirane, A Inomata, T Inoue, N Shishido, I Horii, D Y Loh, K Nakayama
Cell, 85, 707-720 (1996). 8646779

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cdkn1b
MGI:104565		cyclin dependent kinase inhibitor 1B6Cdkn1b<tm1Kin>
MGI:1888790		targeted mutation 1, Kei-ichi Nakayama
  • multiple endocrine neoplasia type 4(MedGEN)
    • neo neomycin resistance gene (E. coli)6 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal Muller cell morphology (MP:0005547)

  • abnormal eye electrophysiology (MP:0005551)

  • abnormal granulosa cell morphology (MP:0008868)

  • abnormal ovarian follicle morphology (MP:0001131)

  • abnormal ovarian folliculogenesis (MP:0001130)
    • more 29 phenotypes
  • abnormal ovulation (MP:0001928)

  • abnormal pituitary intermediate lobe morphology (MP:0008130)

  • abnormal retina outer limiting membrane morphology (MP:0010236)

  • abnormal retina photoreceptor morphology (MP:0001004)

  • abnormal retina pigment epithelium morphology (MP:0005201)

  • abnormal tertiary ovarian follicle morphology (MP:0009363)

  • abnormal thymus apoptosis (MP:0014180)

  • abnormal uterus morphology (MP:0001120)

  • absent corpus luteum (MP:0001134)

  • adrenal medulla hyperplasia (MP:0008301)

  • disorganized retina layers (MP:0001328)

  • endometrium hyperplasia (MP:0009092)

  • enlarged adrenal glands (MP:0000642)

  • enlarged ovary (MP:0004832)

  • enlarged prostate gland (MP:0004958)

  • enlarged scrotum (MP:0001150)

  • enlarged spleen (MP:0000691)

  • enlarged testis (MP:0001148)

  • enlarged thymus (MP:0000709)

  • female infertility (MP:0001926)

  • increased T cell number (MP:0005015)

  • increased amacrine cell number (MP:0008105)

  • increased body size (MP:0001264)

  • increased body weight (MP:0001260)

  • increased pituitary adenoma incidence (MP:0002041)

  • increased pituitary gland weight (MP:0008939)

  • pituitary intermediate lobe hyperplasia (MP:0008371)

  • testicular hyperplasia (MP:0001149)

  • thymus hyperplasia (MP:0000708)
    • Detailed phenotype data

    Ordering Information

    供与核酸mouse phosphoglycerate kinase promoter (PGK promoter), Neomycin resistance gene (neomycin phosphotransferase II (nptII) gene of the transposon Tn5 in Escherichia coli K12), mouse PGK polyA, mouse Cdkn1b genomic DNA
    Research application
    提供条件利用者は事前に寄託者の提供承諾書を得る。共同研究
    Depositor中山　敬一（国立大学法人九州大学）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data