Strain Data Sheet

RBRC00458

Strain Information

Image
BRC No.RBRC00458
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Cdkn1b<tm1Kin>
Former Common nameB6.129P2-Cdkn1b<tm1Kin>; p27 Knockout mice
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Keiko Nakayama and Kei-ichi Nakayama, Howard Hughes Medical Institute, Washington University School of Medicine in 1996. E14 ES cells were used to generate the mutant mice. The mutant mice were backcrossed to C57BL/6.
Strain descriptionp27 (Cdkn1b) gene knockout mice. A 2.5 kb region containing exons 1 and 2 was replaced with a PGK-neo cassette.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Cell, 85, 707-720 (1996). 8646779

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cdkn1b
MGI:104565		cyclin dependent kinase inhibitor 1B6Cdkn1b<tm1Kin>
MGI:1888790		targeted mutation 1, Kei-ichi Nakayama
  • multiple endocrine neoplasia type 4(MedGEN)
    • neo neomycin resistance gene (E. coli)6 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal Muller cell morphology (MP:0005547)

  • abnormal eye electrophysiology (MP:0005551)

  • abnormal granulosa cell morphology (MP:0008868)

  • abnormal ovarian follicle morphology (MP:0001131)

  • abnormal ovarian folliculogenesis (MP:0001130)
    • more 29 phenotypes
  • abnormal ovulation (MP:0001928)

  • abnormal pituitary intermediate lobe morphology (MP:0008130)

  • abnormal retina outer limiting membrane morphology (MP:0010236)

  • abnormal retina photoreceptor morphology (MP:0001004)

  • abnormal retina pigment epithelium morphology (MP:0005201)

  • abnormal tertiary ovarian follicle morphology (MP:0009363)

  • abnormal thymus apoptosis (MP:0014180)

  • abnormal uterus morphology (MP:0001120)

  • absent corpus luteum (MP:0001134)

  • adrenal medulla hyperplasia (MP:0008301)

  • disorganized retina layers (MP:0001328)

  • endometrium hyperplasia (MP:0009092)

  • enlarged adrenal glands (MP:0000642)

  • enlarged ovary (MP:0004832)

  • enlarged prostate gland (MP:0004958)

  • enlarged scrotum (MP:0001150)

  • enlarged spleen (MP:0000691)

  • enlarged testis (MP:0001148)

  • enlarged thymus (MP:0000709)

  • female infertility (MP:0001926)

  • increased T cell number (MP:0005015)

  • increased amacrine cell number (MP:0008105)

  • increased body size (MP:0001264)

  • increased body weight (MP:0001260)

  • increased pituitary adenoma incidence (MP:0002041)

  • increased pituitary gland weight (MP:0008939)

  • pituitary intermediate lobe hyperplasia (MP:0008371)

  • testicular hyperplasia (MP:0001149)

  • thymus hyperplasia (MP:0000708)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), Neomycin resistance gene (neomycin phosphotransferase II (nptII) gene of the transposon Tn5 in Escherichia coli K12), mouse PGK polyA, mouse Cdkn1b genomic DNA
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
    DepositorKeiichi Nakayama (Kyushu University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data