Strain Data Sheet

RBRC00457

Strain Information

Image
BRC No.RBRC00457
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Prkcd<tm1Kin>
Former Common namePKCδ Knockout mice
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain development＞10世代　C57BL/6に戻し交配
Strain descriptionProtein kinase C (PKC) delta is a member of PKC family which phosphorylates a wide variety of protein targets and is known to be involved in diverse cellular signaling pathways. In PKC family members, PKC delta has a unique function to supress cell proliferation. PKC-delta deficient mice exhibit expansion of the B- lymphocyte population and show autoimmune disease after 6 months of age.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
ReferencesNature, 416, 865-869 (2002). 11976687

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Prkcdprotein kinase C, delta14Prkcd<tm1Kin>targeted mutation 1, Kei-ichi NakayamaD14Ertd420e, PKC[d], Pkcd, PKCdelta

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)14neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

供与核酸PGK promoter (mouse phosphoglycerate kinase gene promoter), Neomycin resistance gene (neomycin phosphotransferase II (nptll) gene of the transposon Tn5 in Escherichia coli K12), PGK polyA (mouse phosphoglycerate kinase gene terminater), mouse Prkcd genomic DNA
Research application
提供条件条件を付加する。利用者は事前に寄託者の提供承諾書を得る。
共同研究
Depositor中山　敬一（国立大学法人九州大学）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

Chichger H, Grinnell KL, Casserly B, Chung CS, Braza J, Lomas-Neira J, Ayala A, Rounds S, Klinger JR, Harrington EO.
Genetic disruption of protein kinase Cδ reduces endotoxin-induced lung injury.
Am J Physiol Lung Cell Mol Physiol 303(10) L880-8(2012) 22983354